肿瘤血管内皮细胞的体外模拟培养  

Establishment of in vitro Co-cultue System for Tumor Vascular Endothelial Cells

作　　者：卢松[1] 陈婷婷[2] 别明江[2] 吴家玉[1] 熊中华[1] 

机构地区：[1]四川省肿瘤医院ICU,成都610041 [2]四川大学华西公共卫生学院,成都610041

出　　处：《四川大学学报（医学版）》2013年第2期308-312,共5页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(No.10802054)资助

摘　　要：目的探讨一种肿瘤血管内皮细胞(TECs)体外模拟培养模型,为后续实验做准备。方法利用半透膜孔径为1.0μm的间接共培养双层培养皿建立肿瘤细胞株A549、内皮细胞(HUVECs)体外共培养模型,模拟TECs体内生长环境,对共培养细胞的形态、生长特性、增殖力及染色体核型、端粒酶活性等遗传特性进行初步分析。结果在共培养条件下,共培养A549-HUVECs细胞呈游走迁徙状态,细胞能量代谢旺盛,细胞通透性增加,细胞增殖能力持续增加,处于自稳态调节紊乱所导致细胞增殖与凋亡的平衡失调的状态,细胞染色体核型表现为亚三倍体,端粒酶活性增高,发生表型转化,从而表现为类似癌前病变的病理特征。结论处于体外模拟共培养模型中的A549-HUVECs细胞所发生的特征性变化,类似于体内肿瘤组织中血管内皮细胞的状态,为我们所需要的TECs,说明肿瘤血管内皮细胞的体外模拟培养方法是可行的。Objective To establish a in vitro culture model of tumor vascular endothelial cells （TECs）. Methods We established in vitro culture model of TECs, named A549-HUVECs, through the co-culutre system culturing primary human umbilical vein endothelial cells （HUVECs） and human lung adenocarcinoma epithelial cell line A549 cells in indirect co-cultures, six-well Transwell-clear dishes（0. 4 um porous filter, Costar）. We then observed TECs＇ cell morphology, growth characteristics, proliferation and karyotype, telomerase activity, etc. Results In the indirect co-culture system, A549-HUVECs showed migration cell-type appearance, more active cellular metabolism, increasing cell permeability, augmenting cell proliferation, which indicated A549-HUVECs being in a state of the regulation of homeostasis imbalance for cell proliferation and apoptosis. Besides, the analysis of A549-HUVECs genetic characteristics showed that the chromosome karyotype o~ A549-HUVECs was sub- triploid, and its telomerase activity increased, phenotypic change occurred, which possessed the similar pathological features of precancerous lesion. Conclusion A549-HUVECs co-culture system could be feasible for tumor vascular research.

关 键 词：肿瘤血管内皮细胞 共培养模型 染色体核型 端粒酶活性 

分 类 号：R73-3[医药卫生—肿瘤]