肺腺癌细胞中EGFR蛋白的表达与细胞内胶原化的相关性研究  

Correlation between protein expression of epidermal growth factor receptors and intra-cellular matrix collagenation in lung adenocarcinoma cells

作　　者：李乡南[1] 张丽娜[1] 李宝岩[1] 李建华[2,3] 

机构地区：[1]辽宁中医药大学教学实验中心 [2]中国医科大学附属第一医院病理科 [3]中国医科大学基础医学院病理教研室,沈阳110001

出　　处：《中国组织化学与细胞化学杂志》2013年第1期71-74,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：中国博士后科学基金资助(20060390301);国家自然科学基金资助(30600341)

摘　　要：目的探讨表皮生长因子受体(epidermal growth factor receptor,EGFR)在肺腺癌细胞中的表达及与细胞发生胶原化的相关性。方法从胸水中提取肺腺癌细胞为研究对象,以32例良性胸水中的增生上皮细胞、炎性细胞为对照,采用免疫细胞化学方法检测细胞中EGFR、E钙粘素蛋白、Vimentin、TTF-1和胶原蛋白亚型I的表达。Masson染色方法检测胶原纤维表达。结果 78例胸水标本中,EGFR在肺腺癌细胞中的阳性率为79.5%,胶原蛋白亚型I为32.1%,Masson染色的阳性率为70.5%,明显高于对照组且EGFR和Masson染色的阳性表达结果的相关性具有统计学意义(P<0.01)。结论 EG-FR在肺腺癌细胞中阳性表达,可能与细胞内基质胶原蛋白形成有关。] Objective To investigate the clinical significance of the relationship between EGFR （epidermal growth factor receptor） and intra-cellular matrix collagenation in lung adenocarcinoma cells. Methods Lung adenocarcinoma cells were obtained. 32 cases of hyperplasia epithelial cells of non-malignant pleural effusion were obtained as control. Immunocytoehemiscal staining was used to test EGFR, E- cadherin, Vimentin, TTF-1 and Collagen I. Collagen was analyzed by Masson staining. Results EGFR positive expression was found in 62 of 78 （79. 5%） cases of malignant pleural effusion. Positive rate of Collagen I and Masson staining was 32. 1% and 70.5%. Our data showed that elevated levels of EGFR were accompanied by collagen expression in lung adenocarcinoma cells. Hyperplasia epithelial cells from non-malignant pleural fluid were uniformly negative for EGFR. Conclusion Our study shows EGFR positive expression in lung adenocarcinoma cells may be related to collagen formation in the cytoplasm.

关 键 词：表皮生长因子受体 上皮间质转化 肺腺癌细胞 胸水 

分 类 号：R734.2[医药卫生—肿瘤]