实验性肝纤维化时MMP-1、TIMP-1的表达与Ⅰ、Ⅲ型胶原含量变化的关系  

Relativity of Expression of MMP-1、TIMP-1 and Variability of Type Ⅰ、Ⅲ Collagen During Experimental Liver Fibrosis

作　　者：杨长青[1] 胡国龄[1] 谭德明[1] 张铮 

机构地区：[1]湖南医科大学湘雅医院传染病研究所,湖南长沙410008

出　　处：《临床肝胆病杂志》2000年第4期222-224,共3页Journal of Clinical Hepatology

摘　　要：为了观察实验性肝纤维化时MMP - 1、TIMP - 1的表达与Ⅰ、Ⅲ型胶原含量变化之间的关系 ,本文制备了免疫性大鼠肝纤维化模型 ,运用定量RT -PCR检测肝脏内MMP - 1、TIMP - 1的表达 ,通过免疫组化检测肝组织中Ⅰ、Ⅲ型胶原的含量。运用相关理论作统计学分析。结果发现在肝纤维化发生时 ,Ⅰ型胶原和Ⅲ型胶原含量的变化有显著相关性 ;MMP - 1的表达在肝纤维化组与正常对照组之间未见显著差异 ,与Ⅰ、Ⅲ型胶原的含量变化之间也未见有相关性 ;TIMP - 1的表达在肝纤维化发生时显著增高 ,与Ⅰ、Ⅲ型胶原的变化也有显著相关性。结果表明肝纤维化发生时MMP - 1的表达未见明显改变 ,而TIMP - 1的表达显著增高 ,且与Ⅰ、Ⅲ型胶原含量的增高呈显著相关 ,提示TIMP - 1的表达增高在肝纤维化的发生。To observe the relativity of expression of MMP-1、TIMP-1 and variability of type Ⅰ and Ⅲ collagen during experimental liver fibrosis,we made the rats liver fibrosis model by immune attack of human serum albumin(HSB),and detected the expression of MMP-1 and TIMP-1 by quantitative RT-PCR,assayed the type I and type Ⅲ collagen by immunohistochemistry,then analyzed relativity of expression of MMP-1、TIMP-1 and variability of typeⅠ、Ⅲ collagen during liver fibrosis We found that the change of content of type Ⅰ and type Ⅲ collagen presented a high relativity(r=0 875,P<0 001);no difference was found between normal group and fibrosis group in the expression of MMP-1,and no relativity was found between the expression of MMP-1 and the change of content of type Ⅰ、type Ⅲ collagen;but we found the expression of TIMP-1 became significantly high after formation of liver fibrosis(P<0 01),and it has the high relativity to the variability of type Ⅰand Ⅲ collagen So we got the conclusion that the expression of MMP-1 has no significant change during liver fibrosis,but that of TIMP-1 become obvious high,and there is a distinct relativity between the expression of TIMP-1 and the increasing of type Ⅰ、type Ⅲ collagen during liver fibrosis All of these suggest that increasing of the expression of TIMP-1 play a important role in the formation of liver fibrosis

关 键 词：肝纤维化 基质金属蛋白酶-1 TIMP-1 Ⅰ型胶原 

分 类 号：R575.202[医药卫生—消化系统]