水通道蛋白1在大鼠正常骨骼肌中的表达  

Expression of aquaporin 1 in the rats' skeletal muscle

作　　者：邵先舫[1] 李前[1] 丁青[1] 李莹[1] 严望[2] 

机构地区：[1]湖南中医药大学附属常德医院,常德415000 [2]湖南中医药大学研究生院,长沙410007

出　　处：《中华中医药杂志》2013年第3期819-820,共2页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：湖南省自然科学基金项目(No.10JJ6038)~~

摘　　要：目的:观察水通道蛋白1(AQP1)在SD大鼠正常骨骼肌中的表达,为深入探讨AQP1在大鼠骨骼肌损伤后肿胀状态下的表达及生理作用奠定基础。方法:收集8只6月龄SD大鼠左后肢外侧股四头肌肌群中约200mg肌肉组织,运用实时荧光定量PCR、Western blot检测AQP1在大鼠肌肉组织中的表达。结果:实时荧光定量PCR显示AQP1 mRNA在肌肉组织中稳定表达;Western blot检测显示肌肉组织中AQP-1在28KD左右有一特异性条带。结论:AQP1在SD大鼠正常肌肉组织中的稳定表达提示其可能参与肌肉组织内的液体平衡,对维持肌肉组织的正常生理功能、在创伤性肌肉肿胀的发病机制中可能有重要作用。Objective： To determine the expression of aquaporinl（AQP1） in the SD rats＇ skeletal muscle. For the thorough discussion the expression of AQP1 in rat skeletal muscle swelling state and physiological role laying the foundation. Methods： About 200mg muscle tissue（8 cases） were collected from rats＇ left hind limb lateral quadriceps femoris muscle group. The expression of AQP1 was detected by real-time quantitative PCR and Western blot. Results： Real-time quantitative PCR showed AQP1 mRNA was expressed in skeletal muscle. Western blot revealed there was a specific band at the site of around 28KD. Conclusion： The expression of AQP1 in rats＇ skeletal muscle suggests that it may be involved in the liquid balance of muscle tissue, and plays an important role in maintaining muscle tissue of normal physiological function, in the pathogenesis of traumatic muscle swelling.

关 键 词：水通道蛋白1 骨骼肌 液体平衡 

分 类 号：R363[医药卫生—病理学]