通过HSQC和~1H,^(13)C-COSY叠合增强二维谱分辨率的方法  

A Method for Improving the Resolution of Two-Dimensional NMR Spectrum by Overlapping HSQC and ~1H,^(13)C-COSY

作　　者：朱传钧[1,2] 崔育新[3] 姜标[1,4] 

机构地区：[1]中国科学院上海高等研究院,上海201210 [2]上海精细化工火炬创新创业园分析中心,上海201512 [3]天然药物及仿生药物国家重点实验室(北京大学),北京100191 [4]中国科学院上海有机化学研究所,上海200032

出　　处：《波谱学杂志》2013年第1期86-92,共7页Chinese Journal of Magnetic Resonance

基　　金：国家自然科学基金(重点项目)资助项目(20832007);国家科技重大专项(重大新药创制)资助项目(2009ZX09501-016;2009ZX09501-007)

摘　　要：通常二维谱F1维分辨率较差,F2维分辨率较好.即HSQC实验13 C维度分辨率较差,1 H维度分辨率较好.而1 H,13 C-COSY实验13 C维度分辨率较好,1 H维度分辨率较差.提出一种通过HSQC和1 H,13 C-COSY叠合,取较弱的值储存并显示的方法增强分辨率,采用Bruker Topspin软件AU程序实现.该方法在不明显增加采样时间的情况下,可显著增强分辨率,信噪比也没有降低.同时抑制了F1噪声.处理后,谱图美观清晰.In traditional two-dimensional （2D） NMR experiments, spectral resolution in the F, dimension is usually higher than that in the F2 dimension. Therefore, in the HSQC experiments, the spectral resolution in the ^13C dimension is lower than that in the ^1H dimension. This is the opposite in the ^1H, ^13C-COSY experiments. In this work, a method for improving the resolution of 2D NMR spectrum was presented, in which HSQC and ^1H, ^13C-COSY spectra were overlapped, and then the weaker points were selected, saved and displayed. The method was implemented as an AU program within the Bruker Topspin framework. It was demonstrated experimentally that the method could improve spectral resolution, at no cost of signal-to-noise ratio. The meth-od can also be used to suppress F1 noise.

关 键 词：核磁共振(NMR) 分辨率 AU程序 叠合 HSQC 1H 13C-COSY 

分 类 号：O482.53[理学—固体物理]