实验性大鼠牙移动过程中TRPV1和IL-1α在牙周膜中的表达  

The change of transient receptor potential vanilloid-1 and interleukin-1α in periodontal tissue during experimental tooth movement

作　　者：高宇男[1] 周洪[1] 朱永进[2] 乔虎[1] 

机构地区：[1]西安交通大学医学院附属口腔医院正畸科,陕西西安710004 [2]西安交通大学医学院附属口腔医院医学研究中心,陕西西安710004

出　　处：《上海口腔医学》2013年第1期1-6,共6页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(30973359);陕西省自然科学基金(2009JQ4007)~~

摘　　要：目的:研究实验性大鼠牙移动过程中,加力不同时间及力值后,香草酸瞬时亚型1(transient receptor potential vanilloid-1,TRPV1)和白细胞介素1α(interleukin-1α,IL-1α)在牙周膜中的表达规律,进而探讨两者在正畸疼痛中的作用。方法:将66只SD大鼠随机分为对照组、假手术组、实验组共11组,每组6只。实验组大鼠施50 g力后,分别于4 h、8 h、1 d(1 d组据力值大小增加30、50、80 g 3个亚族)、3 d、5 d、7 d、14 d,随机处死6只,切取牙周膜组织,采用实时定量PCR技术观察各组大鼠牙周膜内TRPV1和IL-1αmRNA的表达,采用SPSS13.0软件包对数据进行统计学分析。结果:同一力值,牙周膜内TRPV1和IL-1α在加力4 h后升高,1 d时到达峰值,7 d后下降至正常水平。牙周膜内TRPV1和IL-1α的表达随力值增加而升高。结论:在生物机械力的诱导下,牙周膜内TRPV1和IL-1α的表达与加力时间及大小呈现规律性变化,提示TRPV1和IL-1α与正畸疼痛存在一定联系。PURPOSE： To investigate the expression of TRPV1 and IL-lα in Sprague-Dawley rats＇periodontal ligament （PLD） during experimental tooth movement of different orthodontic force and duration, and explore the role of TRPV1 and IL-1α in orthodontic pain. METHODS： 66 Sprague-Dawley rats were divided into control group （n=6）,sham operation group （n=6） and experimental group （n=54） randomly. Orthodontic force was applied on right first maxillary molar in rats and the changes of TRPV1 and IL-lot expression were detected by real-time PCR at 4 h, 8 h, 1 d （three subgroups were added according to different forces： 1 d-30 g, 1 d-50 g, 1 d-80 g ）, 3 d, 5 d, 7 d, 14 d after tooth movement. The data was analyzed using SPSS 13.0 software pakage. RESULTS： Following the experimental tooth movement, the expression of TRPV1 and lL-lα in periodontal tissues were significantly up-regulated from 4 h to 7 d, with a peak at 1 d and returned to normal level at 1 week. The greater force applied during experimental tooth movement at 1 d, the higher expression of TRPV1 and IL-lα were detected in periodontal tissues. CONCLUSIONS： Experimental tooth movement leads to regular change of TRPV1 and IL-lαexpression in periodontal tissues, which indicates that TRPV1 and IL-lα may play an important role in orthodontic pain. Supported by National Natural Science Foundation of China （30973359） and Natural Science Foundation of Shanxi Province （2009JQ4007）.

关 键 词：正畸疼痛 实时定量PCR 瞬时受体电位香草素亚型1 白细胞介素1Α 

分 类 号：R783.5[医药卫生—口腔医学]