3种牙科金属材料对L929细胞的毒性及对凋亡相关基因与蛋白表达的影响  

Effect of three dental alloys on cytotoxicity and apoptosis related gene expression in L929 cells

作　　者：孟贺[1] 李秀梅[2] 徐艳丽[1] 梁锐英[1] 吴文慧[1] 

机构地区：[1]河北联合大学口腔医学院口腔修复科,河北唐山063000 [2]辽宁医学院附属第二医院口腔修复科,辽宁锦州121000

出　　处：《上海口腔医学》2013年第1期30-34,共5页Shanghai Journal of Stomatology

基　　金：唐山市科技局指令性项目(12140209A-39);河北联合大学青年基金(Z201142)~~

摘　　要：目的:研究3种牙科金属材料(金合金、纯钛、镍铬合金)浸提液对小鼠成纤维细胞L929的细胞毒性及凋亡相关基因与蛋白表达的影响,探讨3种牙科金属材料引起细胞凋亡的可能通路。方法:以金合金(A组)、纯钛(B组)、镍铬合金(C组)的浸提液(每组8个样本)培养小鼠成纤维细胞L929,以含10%胎牛血清的RPMI1640培养基作为阴性对照(D组),以铜合金作为阳性对照(E组)。采用MTT法在细胞水平评价3种牙科金属材料的细胞毒性,采用RT-PCR在分子水平上检测3种牙科金属材料浸提液对L929细胞凋亡相关基因caspase-3、-8、-9 mRNA及蛋白表达的影响。采用SPSS11.5软件包对数据进行统计学分析。结果:各实验组细胞毒性均为0级。镍铬合金组caspase-3mRNA与caspase-9mRNA的表达与其他实验组、阴性对照及阳性对照组间差异显著,镍铬合金组caspase-3蛋白与caspase-9蛋白的表达与其他实验组及阴性对照组间差异显著,各组caspase-8mRNA及蛋白的表达无显著差异。结论:金合金与纯钛浸提液对小鼠L929细胞凋亡相关基因及蛋白的表达无显著诱导作用;镍铬合金诱导小鼠L929细胞凋亡相关基因caspase-3mRNA、caspase-9mRNA及蛋白表达增加,可能通过线粒体通路诱导细胞凋亡。PURPOSE：To investigate the effect of the liching liquids of 3 different kinds of dental alloys on L929 cells both at cell level and molecular level. METHODS： The fibroblast L929 cells of mouse were treated in vitro with leaching liquids of 3 different kinds of dental alloys which were Au alloy, Ti and Ni-Cr alloy, respectively. The RPMI 1640 cell medium containing 10% fetal calf serum was served as a negative control and the Cu alloy was served as a positive control. The cytotoxicities of 3 dental alloys were evaluated by means of MTT and the effects of these alloys on the expression of caspase- 3, caspase-8 and caspase-9 of L929 cells were examined by RT-PCR method and immunohistochemistry. The data was analyzed with SPSS13.0 software package. RESULTS： The cytotoxicity of three groups were all in grade 0 after 48 hours of cultures. Caspase-8 had no change in all groups at mRNA level.The expression of caspase-3 and caspase-9 in Ni-Gr alloy group was significantly different from other groups. CONCLUSIONS： It is suggested from the results that the leaching liquids of Ni-Cr alloy may induce cell apoptosis through mitochondrion pathway. Supported by Prescriptive Project of Bureau of Science and Technology of Tangshan City（12140209A-39） and Youth Fund of Hebei United University（Z201142）.

关 键 词：牙科金属 浸提液 成纤维细胞L929 凋亡 

分 类 号：R783.1[医药卫生—口腔医学]