牙周牙髓联合病变牙周袋内厌氧菌的培养检测和药敏试验  

Detection of anaerobes and drug sensitivity from the periodontal pockets of patients with combined periodontal-endodontic lesions

作　　者：周康 季佩红 俞立英[2] 陈琼[2] 许全林 

机构地区：[1]上海市杨浦区牙病防治所,上海200090 [2]复旦大学附属华山医院口腔科,上海200040

出　　处：《上海口腔医学》2013年第1期72-76,共5页Shanghai Journal of Stomatology

基　　金：杨浦区卫生局医学重点专科项目(2009AA02-09)~~

摘　　要：目的:分析牙周牙髓联合病变牙周袋内厌氧菌的分布及药敏情况,为临床治疗提供理论依据。方法:从82例牙周牙髓联合病变的深牙周袋内分离培养得到86株厌氧菌,采用琼脂稀释法进行6种抗菌药物的药敏试验。结果:牙周牙髓联合病变的牙周袋内厌氧菌的检出率高,主要包括消化链球菌及链球菌、普雷沃菌、梭杆菌、放线菌、韦荣菌等。其中革兰阴性菌占53.4%,革兰阳性菌占46.5%。牙周源组与牙髓源组所检出的厌氧菌有明显差异。厌氧菌对各种抗菌药物敏感,其中对青霉素、克林霉素、甲硝唑的耐药率分别为6.9%、13.9%和2.3%。结论:厌氧菌与牙周牙髓联合感染的关系密切,临床上应选择敏感药物进行治疗。PURPOSE： To investigate the correlation between the composition of bacterial flora isolated from deep periodontal pockets with combined periodontal-endodontic lesions, and to test the antibiotic susceptibility of anaerobes. METHODS： Microbial samples were taken from 82 deep periodontal pockets with combined periodontal-endodontic lesions using adequate techniques, and divided into two groups： endo-perio group and perio-endo group. Antibiotic susceptibility of cultivable anaerobes were tested with agar dilution method. RESULTS： Eighty-six cultivable anaerobes were isolated, including Streptococcus spp, Prevotella spp, Actinomycetes spp, VeilloneUa spp, Fusobacterium spp and etc. 53.4% were gram-positive anaerobes, while 46.51% were gram-negative anaerobes. The detection rate of anaerobes in endo-perio group and that in perio-endo group had significant difference. Most isolated anaerobes were sensitive to six drugs. The resistance rates of penicillin, clindamycin, metronidazole were 6.8%, 13.9% and 2.2%. CONCLUSIONS： There is a close relationship between anaerobes and combined periodontal-endodontic lesions. Drug sensitive susceptibility should be detected prior to clinical treatment of combined periodontal-endodontic lesions. Supported by Key Medical Discipline Project of Bureau of Health of Yangpu District （2009AA02-09）.

关 键 词：牙周牙髓联合病变 厌氧菌 药敏试验 

分 类 号：R780.2[医药卫生—口腔医学]