Effects of resveratrol on hydrogen peroxide-induced oxidative stress in embryonic neural stem cells  

作　　者：Sibel Konyalioglu Guliz Armagan Ayfer Yalcin Cigdem Atalayin Taner Dagci 

机构地区：[1]Department of Biochemistry,Faculty of Pharmacy,Ege University [2]Department of Restorative Dentistry and Endodontics,Faculty of Dentistry,Ege University [3]Department of Physiology,School of Medicine,Ege University [4]Center for Brain Research,Ege University

出　　处：《Neural Regeneration Research》2013年第6期485-495,共11页中国神经再生研究（英文版）

基　　金：funded by the Research Fund of Ege University,Project No. 05/ECZ/020

摘　　要：Resveratrol, a natural phenolic compound, has been shown to prevent cardiovascular diseases and cancer and exhibit neuroprotective effects. In this study, we examined the neuroprotective and antJoxJdant effects of resveratrol against hydrogen peroxide in embryonic neural stem cells. Hydrogen peroxide treatment alone increased catalase and glutathione peroxidase activities but did not change superoxide dismutase levels compared with hydrogen peroxide ＋ resveratrol treatment. Nitric oxide synthase activity and concomitant nitric oxide levels increased in response to hydrogen peroxide treatment. Conversely, resveratrol treatment decreased nitric oxide synthase activity and nitric oxide levels. Resveratrol also attenuated hydrogen peroxide-induced nuclear or mitochondrial DNA damage. We propose that resveratrol may be a promising agent for protecting embryonic neural stem cells because of its potential to decrease oxidative stress by inducing higher activity of antioxidant enzymes, decreasing nitric oxide production and nitric oxide synthase activity, and alleviating both nuclear and mitochondrial DNA damage.Resveratrol, a natural phenolic compound, has been shown to prevent cardiovascular diseases and cancer and exhibit neuroprotective effects. In this study, we examined the neuroprotective and antJoxJdant effects of resveratrol against hydrogen peroxide in embryonic neural stem cells. Hydrogen peroxide treatment alone increased catalase and glutathione peroxidase activities but did not change superoxide dismutase levels compared with hydrogen peroxide ＋ resveratrol treatment. Nitric oxide synthase activity and concomitant nitric oxide levels increased in response to hydrogen peroxide treatment. Conversely, resveratrol treatment decreased nitric oxide synthase activity and nitric oxide levels. Resveratrol also attenuated hydrogen peroxide-induced nuclear or mitochondrial DNA damage. We propose that resveratrol may be a promising agent for protecting embryonic neural stem cells because of its potential to decrease oxidative stress by inducing higher activity of antioxidant enzymes, decreasing nitric oxide production and nitric oxide synthase activity, and alleviating both nuclear and mitochondrial DNA damage.

关 键 词：neural regeneration traditional Chinese medicine stem cells RESVERATROL embryonic neural stemcells hydrogen peroxide CATALASE glutathione peroxidase nitric oxide synthase nitric oxide DNADAMAGE neuroprotection grants-supported paper neuroregeneration 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]