CXCR4基因沉默后对T24膀胱癌细胞株裸鼠移植瘤的影响  

The effect of CXCR4 gene-silencing on the growth of T24 cell line transplantated tumor in Nude Mice

作　　者：郑湘予[1] 庞霞[1] 李晟磊[1] 朱志强[2] 

机构地区：[1]郑州大学第一附属医院病理科河南省肿瘤病理重点实验室,河南郑州450052 [2]郑州大学第一附属医院急诊科,河南郑州450052

出　　处：《中国现代医学杂志》2012年第33期13-18,共6页China Journal of Modern Medicine

摘　　要：目的探讨CXC趋化因子受体4(CXCR4)基因沉默后对人膀胱移行细胞癌裸鼠移植瘤的影响。方法构建人膀胱移行细胞癌T24细胞株裸鼠膀胱癌移植瘤模型,荷瘤裸鼠随机分为小干扰RNA(siRNA)组、空白对照组和阴性对照组,各组分别在瘤旁注射CXCR4 siRNA、无菌生理盐水和无关序列。监测肿瘤生长情况,比较各组裸鼠成瘤大小。采用RT-PCR、免疫组织化学及Western blot方法检测各组裸鼠肿瘤组织中CXCR4 mRNA及蛋白的表达情况。结果 siRNA干扰组裸鼠移植瘤体积显著小于空白对照组和阴性对照组,分别为(1 423.60±148.23)mm3、(2 516.00±208.03)mm3和(2 420.40±162.36)mm3,P<0.05;siRNA干扰组裸鼠移植瘤CXCR4 mRNA水平及蛋白表达率显著低于空白对照组和阴性对照组,分别为(0.397±0.035)vs(0.844±0.027)v(s 0.828±0.015),P<0.05和(62.60±11.89)vs(132.60±13.28)v(s 127.60±16.02),P<0.05。结论CXCR4 siRNA能抑制人膀胱移行细胞癌T24细胞株裸鼠皮下移植瘤的形成,且能在体内有效下调CX-CR4 mRNA和蛋白的表达。[ Objective ] To investigate the effect of chemokine receptor 4 （CXCR4） gene-silencing on the growth of transplantated tumors of bladder transitiomal cell carcinoma in nude mice. [Methods ] Nude mice model bearing bladder transitiomal cell carcinoma was reproduced by implanting human bladder transitiomal cell carcinoma cell line T24. Then they were randomly subdivided into three groups： CXCR4 small interfering RNA （siRNA） was injected to the area nearby the tumors （interference group）, stroke-physiological saline solution and nosense sequence were respectively administrated served as blank control group and negative control group. Tumor growth and tumor size were measured. Histopathological changes of the tumors were observed with HE staining, and the mRNA and protein expressions of CXCR4 were detected by RT-PCR and immunohistochemistry and Western-blot respectively. [ Results ] The volume of transplantated tumor in interference group was significantly smaller than that in blank con- trol group and negative control group （1 423.60±148.23）mm^3 vs （2 516.00±208.03）mm^3 vs （2 420.40±162.36）mm^3; P 〈0.05）; and the relative level of mRNA and the expression of protein of CXCR4 in interference group was significantly lower than that in bank control group and negative control group （mRNA： 0.397±0.035 vs 0.844±0.027 vs 0.828±0.015, P 〈0.05; protein： 62.60±11.89 vs 132.60±13.28 vs 127.60±16.02, P 〈0.05）. [Conclusion] Our results suggest that CXCR4 siRNA could inhibit the tumorigenesis of T24 cell transplantated in nude mice and downregu- late the CXCR4 mRNA and protein expressions in vivo.

关 键 词：CXCR4 膀胱移行细胞癌 RNA干扰 裸鼠 

分 类 号：R737.14[医药卫生—肿瘤]