喉乳头状瘤组织中HPV16、18病毒的检测  

Detection of HPV16,18 in laryngeal papillomatosis tissue

作　　者：鲁孟显[1] 王炳惠 李俊平 李鸿敬 

机构地区：[1]甘肃省人民医院耳鼻咽喉头颈外科,甘肃兰州730000 [2]兰州市第一人民医院耳鼻咽喉头颈外科,甘肃兰州730050 [3]临洮县人民医院耳鼻咽喉头颈外科,甘肃临洮730500 [4]水电四局职工医院耳鼻咽喉科,甘肃永靖731600

出　　处：《兰州大学学报（医学版）》2012年第3期23-26,共4页Journal of Lanzhou University（Medical Sciences）

摘　　要：目的检测喉乳头状瘤组织人乳头状瘤病毒（HPV）DNA含量,探讨HPV感染与喉乳头状瘤的关联性和喉乳头状瘤发病的分子生物学机制。方法应用荧光探针标记引物的定量聚合酶链反应技术对35例喉乳头状瘤组织、20例声带息肉组织中HPV16、18 DNA的含量进行检测。结果 35例喉乳头状瘤组织中HPV16 DNA阳性25例,其DNA模板平均拷贝数为4.89×10~5（1.2×10~3～2.41×10~7）,HPV18DNA阳性8例,其DNA模板平均拷贝数为1.54×10~4（1.09×10~3～5.36×10~5）,其中HPV16、18 DNA均为阳性4例;HPV18 DNA阳性而HPV16 DNA阴性3例;HPV16 DNA阳性而HPV18 DNA阴性18例,其DNA模板平均拷贝数为2.83×10~6（1.2×10~3～2.41×10~7）。20例声带息肉组织HPV16、18 DNA均阴性。二者具有显著性差异（P〈0.01）。结论 HPV16、18感染是喉乳头状瘤致病的重要因素,HPV16 DNA含量与喉乳头状瘤的发生有一定的相关性,荧光定量PCR技术对检测HPV16、18的感染和喉乳头状瘤早期诊断是一种有效方法。Objective To detect quantitation of human papillomavirus（HPV） DNA of laryngeal papillomatosis tissue in patients with current laryngeal papillomatosis,and to explore the relationship between HPV and current laryngeal papillomatosis and the molecular mechanism of laryngeal papillomatosis.Methods Fluorescence quantitative polymerase chain reaction was used for the detection of HPV16,18 DNA in 35 cases of laryngeal papillomatosis and 20 cases of tissues vocal cord polyps.Results 25 tissues out of 35 laryngeal papillomatosis specimens were confirmed to be HPV16 DNA type positive.The average copy number of DNA template in 16 type positive samples was 4.89×10~5（1.2×l0~3～2.41×10~7）.Eight specimens＇ tissues were confirmed to be HPV18 DNA type positive.The average copy number of DNA template in 18 type positive samples was 1.54×l0~4（1.09×10~3～5.36×10~5）.Both HPV16,18 DNA were positive in 4 cases.Three cases＇ HPV18 DNA was positive,but their HPV16 DNA was negative.HPV16 DNA was positive,but HPV18 DNA was negative in 18 cases.The average copy numbers of DNA template in 16 type positive samples was 2.83×10~6（1.2×l0~3～2.41×10~7）.Both HPV16,18 DNA were negative in 20 cases of vocal cord polyps tissue.The difference was markedly significant （P0.01）.Conclusion This study indicates that the infection of HPV16,18 is one of the important factors in the development of laryngeal papillomatosis.The quantitation of HPV16 DNA is associated with laryngeal papillomatosis.Fluorescence quantitative polymerase chain reaction may be an effective method in detection of HPV16,18 infection and early diagnosis of laryngeal papillomatosis.

关 键 词：喉乳头状瘤 人乳头状瘤病毒 荧光定量 

分 类 号：R734.1[医药卫生—肿瘤]