分段式补料批次发酵对凝结芽孢杆菌发酵水平的影响  

The Influence on Fermentation Level of Bacillus coagulans by Adopting Segmented Fed-batch Fermentation Technology

作　　者：高书锋[1] 孔利华[1] 周映华[1] 胡新旭[1] 吴胜莲[1] 缪东[1] 周小玲[1] 刘惠知[1] 

机构地区：[1]湖南省微生物研究所,湖南长沙410009

出　　处：《中国畜牧兽医》2013年第2期80-84,共5页China Animal Husbandry & Veterinary Medicine

基　　金：长沙市科技攻关资金专项(K1003312-21)

摘　　要：试验采用分段式补料批次发酵技术对1株畜禽用凝结芽孢杆菌的发酵水平进行了研究,对数期补料促使菌体量大量积累,稳定期补料促进芽孢大量形成,从而达到高菌体量和高芽孢率的目的。试验结果表明,对数期补加淀粉量为总淀粉量的10%,豆粉和鱼粉(质量比为2∶1)补加量为总豆粉和鱼粉量(质量比为2∶1)的5%,补加方式为2次等量补加(间歇10～12h),发酵水平由分批发酵6.80×109 CFU/mL提高到8.30×109 CFU/mL;稳定期最佳补料浓度为0.10g/L碳酸钙、0.156g/L磷酸二氢钠、0.30g/L蛋氨酸,最佳补料方式为1次性补加,经稳定期补料优化,芽孢率由分批发酵的75.78%提高到85.63%。因此,采用分段式补料批次发酵技术能够进一步提升发酵液的菌体数和芽孢率。The experiment was aimed to research on fermentation level of Bacillus coagulans strain applied for animals by adopting segmented fed-batch fermentation technology. The fed-batch in exponential and stationary phases respectively increased the number of bacterium and promoted spore formation. The results showed in exponential phase that the quantity of starch additives was 10 % of the total,the quantity of soya bean flour and fish meal additives was 5 % of the total,the best fed batch mode was fed twice equally （interval time for 10 to 12 h）,on this conditions the living bacteria amount could reach 8.30×10^9 CFU/mL from 6.80×10^9 CFU/mL. The results showed in stationary phase that the best fed-batch concentration was calcium carbonate 0.10 g/L,sodium dihydrogen phosphate 0. 156 g/L and methionine 0.30 g/L, the best fed-batch mode was one-off feeding,on this conditions the ratio of spore-forming could reach 85. 63% from 75. 78%. Therefore, the research through the experiment on Bacillus coagulans suggested that both the living bacteria and the ratio of spore-forming of fermentation broth further increased by adopting segmented fed-batch fermentation technology.

关 键 词：凝结芽孢杆菌 芽孢率 发酵优化 分段式补料 

分 类 号：S816.6[农业科学—饲料科学]