TBTC对斑马鱼磷酸酶、ATPase和类固醇激素影响的研究  

Effect of TBTC on phosphatase,ATPase and steroid hormones in zebra fish

作　　者：周连宁[1,2,3] 黎双飞[2] 刘芳[2] 吴属连[1,3] 赵振业[1,3] 

机构地区：[1]深圳市海岸与大气研究重点实验室,广东深圳518057 [2]深圳大学生命科学学院,广东深圳518060 [3]深港产学研环保工程技术股份有限公司,广东深圳518057

出　　处：《广东农业科学》2013年第1期119-122,共4页Guangdong Agricultural Sciences

基　　金：广东省农业科技计划项目(2008B020800003);深圳市创新环境建设项目(ZDSY20120618162015166);深圳市生物产业发展专项资金(JC201005280675A)

摘　　要：采用不同浓度的三丁基氯化锡(TBTC)处理斑马鱼一定时间后,对其体内磷酸酶、三磷酸腺苷酸酶(ATPase)活性及性腺类固醇激素变化进行检测。结果表明,与对照组相比,试验组斑马鱼体内碱性磷酸酶(AKP)和酸性磷酸酶(ACP)活性均显著性升高,但头部和内脏匀浆液各浓度组AKP和ACP活性增高程度没有表现出明显的剂量依赖关系;Ca2+-ATPase和Na+/K+-ATPase活性均受到了明显的抑制作用,且TBTC浓度越高,受抑制程度越明显;性类固醇激素也有明显的差别,其中雌性性腺的睾酮含量增加明显,而雄性性腺的雌二醇含量明显下降。由此可知,TBTC可对生物体产生显著的生理影响和毒害作用,且对生物个体的毒害作用和影响是多方面的,低剂量长期暴露同样会产生显著的生理毒害效应。After treated with different concentrations of TBTC, the changes of phosphatase, ATPase activity and gonad steroid hormones of zebra fishes were studied. The result showed that compared with the control group, the activities of phosphatase （AKP and ACP） in the fishes of experimental groups were all significantly increased, but the increased level of AKP and ACP activity in head and viscera homogenate didn＇t show strong dose dependent. The activities of Ca2^-ATPase and Na＋/K^-ATPase were significantly restrained, the higher the concentration of TBTC, the more obvious the restraining effect. Sex steroid hormones also showed great differences, the content of testosterone in female gonad increased significantly while the content of estradiol in male gonad decreased a lot. The results dicated that there were significant physiological effects and toxicity from TBTC on the organisms, and toxic effects and impact on life individuals were quite extensive, long-term exposured to low doses TBTC would also cause significant physiological toxic effects.

关 键 词：三丁基氯化锡(TBTC) 磷酸酶 ATPASE 类固醇激素 

分 类 号：X503.2[环境科学与工程—环境工程]