雷州黑鸭p94部分序列的克隆与蛋白功能分析  

Clone and protein function analysis of p94 partial sequence of Leizhou black duck

作　　者：汤绮明[1] 苏瑛[1] 张丽[1] 刘丽文[1] 陈琦[1] 马猛[1] 黄骏腾[1] 刘鸿[1] 

机构地区：[1]广东海洋大学农学院,广东湛江524088

出　　处：《广东农业科学》2013年第1期147-149,153,共4页Guangdong Agricultural Sciences

基　　金：广东省自然科学基金(06029116);广东省科技攻关项目(2010B020311012);广东海洋大学博士启动基金(1212040);湛江市科技计划项目(2012C02)

摘　　要：采用RT-PCR法对雷州黑鸭p94部分序列进行克隆,获得了大小为1 100 bp的核苷酸序列,该序列编码351个氨基酸,其中亲水性氨基酸所占比例较高。对其蛋白结构进行分析,发现不存在跨膜结构及信号肽,其蛋白结构与鸡的p94分子蛋白结构相似。与人、鼠、鸡等物种进行同源性比对并构建系统发育树,结果发现,雷州黑鸭与鸡的同源性高达93.8%,在系统发育树中也聚为一支。综合分析可以推断出,雷州黑鸭的p94蛋白功能与鸡相似,参与肌纤维的发育及肌肉嫩度的调控,这为其在肉质嫩度上的表达调控提供了理论基础。In this research, p94 gene with 1 100 bp nucleotide was cloned from a Leizhou black duck by RT-PCR. It encoded a peptide with 351 amino acids, including a high proportion of hydrophilic amino acid. Through analysis on protein structure, it found that there was no transmembrane and signal peptide, which was similar to chicken＇s p94 protein structure. Compared with human, mouse, chicken and other species on sequence identity, Leizbou black duck was 93.8% similar to that of chicken. And in phylogenetic tree, Leizhou black duck and chicken were in the same branch. In a word, it infered that p94 protein function of Leizhou duck was similar to that of chicken which participate in the development of muscle fibers and regulation of muscle tenderness. This provides a theoretical basis for expression and regulation of p94 on meat tenderness.

关 键 词：雷州黑鸭 p94 克隆 蛋白功能分析 

分 类 号：Q781[生物学—分子生物学]