不同剂量乌司他丁对离体兔肺保护作用的实验研究  

Explore the Protective Effect of Different Doses of Ulinastain on the Isolated Lung

作　　者：戴宁凰 骆金华[2] 陈伟[1] 郑中锋 臧其威[1] 

机构地区：[1]南京鼓楼医院集团宿迁市人民医院胸外科,江苏省宿迁市223800 [2]南京医科大学第一附属医院胸心外科

出　　处：《实用心脑肺血管病杂志》2013年第2期43-44,共2页Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease

摘　　要：目的研究不同剂量乌司他丁对离体兔肺的保护作用。方法选择36只新西兰大白兔作为研究对象,采用随机分组的方法,分为A、B、C 3组,各12只。A组给予UW液,为对照组;B组给予UW液+乌司他丁2万U/kg;C组给予UW液+乌司他丁5万U/kg。切取实验兔整体心肺作为离体心肺模型,分别在2h末和4h末,取肺部分标本做电镜分析,4h末取右肺检测测肺气道压力(Paw)、肺湿/干重比(W/D),用负压吸引真空试管取左心房血液做血气分析。结果 3组4h后Paw、W/D、二氧化碳分压(PCO2)、氧分压(PO2)比较,差异均有统计学意义(P<0.05)。B组、C组Paw、W/D、PCO2、PO2与对照组比较,差异均有统计学意义(P<0.01);C组上述指标与B组比较,差异有统计学意义(P<0.05)。超微结构分析:B组和C组的Ⅰ型细胞形态稍好于A组,C组稍好于B组。结论乌司他丁对离体肺的保护具有显著作用,大剂量乌司他丁对离体兔肺保护作用更明显。Objective To investigate the protective effect of different doses of Ulinastain perfusion on the isolated lung. Methods 36 rabbits were randomly divided into A, B, C 3 groups, each 12 rabbits. Group A were given UW fluid as control group; group B were given UW fluid with Ulinastain 20000U/kg; group C were given UW fluid with Ulinastain 50000U/ kg group. The lung specimens were obtained in order to analyze the change in morphology by using electron microscope at 2 and 4 hours after perfusion. Detect the ratio of wet/dry weight （W/D） ; airway pressure （Paw） of lung model; gas analysis of the blood which was pumped into the lung model after 4 hours of perfusion. Results 3 groups of 4h after Paw, W/D, partial pressure of carbon dioxide （ PCO2 ） , oxygen partial pressure （ PO2 ） , the differences were statistically significant （ P 〈 0. 05 ）. In group B, group C Paw, W/D, PCO2, PO2 compared with the control group, the difference was statistically significant （P 〈 0. 01 ）; C group compared with B group, the difference was statistically significant （P 〈 0. 05）. Ultrastructural analysis： B group and C group of type I cell morphology was slightly better than A group, C group better than B group. Conclusion Ulinastatin has a significant role in the protection of isolated lung, the lung protective function is the positive correlation with the dosage, high - dose ulinastatin has more pronounced isolated rabbit lung protective effect.

关 键 词：肺灌注 兔 肺 乌司他丁 动物 实验 

分 类 号：R-332[医药卫生]