莫诺苷对局灶性脑缺血再灌注大鼠凝血四项的影响  

Effect of Morroniside on Coagulation in Focal Cerebral Ischemia/Reperfusion in Rats

作　　者：袁廷伟[1,2] 孙芳玲[2] 王晓峰[2] 艾厚喜[2] 王文[2] 龚大春[1] 

机构地区：[1]三峡大学,湖北宜昌市443002 [2]首都医科大学宣武医院药物研究室,北京市100053

出　　处：《中国康复理论与实践》2013年第2期104-106,共3页Chinese Journal of Rehabilitation Theory and Practice

基　　金：科技部"十二五"重大创新药专项(No.2012ZX09102-201-016);国家自然科学基金(No.81173575);国家自然科学基金(No.30740053);北京市自然科学基金(No.7062031);北京市教委科研基地-科技创新平台(No.111219);北京市卫生系统高层次卫生技术人才(No.2011-3-097)

摘　　要：目的研究莫诺苷对局灶性脑缺血再灌注大鼠纤维蛋白原(Fib)、凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)和凝血酶时间(TT)的影响。方法用线栓法制备大鼠局灶性脑缺血再灌注模型,造模后,分别给予莫诺苷低剂量(30 mg/kg)、中剂量(90 mg/kg)、高剂量(270 mg/kg)连续灌胃7 d,阿司匹林(ASA)为阳性对照药。使用试剂盒分别检测Fib、PT、APTT、TT指标的变化。结果与假手术组相比,模型组Fib含量显著增加(P<0.001),PT、APTT、TT显著缩短(P<0.001);与模型组相比,莫诺苷能明显降低Fib含量(P<0.01),延长PT、APTT、TT(P<0.05)。结论莫诺苷具有对抗局灶性脑缺血再灌注大鼠凝血功能的作用。Objective To investigate the effects of morroniside on fibrinogen （Fib）, prothrombin time （PT）, activated partial thrombo- plastin time （APTT） and thrombin time （TT） in focal cerebral ischemia/reperfusion in rats. Methods The animal model was induced with oc- clusion of middle cerebral artery （MCAO） with suture embolus, and morroniside was then administered intragastrically at the dose of 30, 90 and 270 mg/kg for 7 d. Acetyl salicylic acid was taken as positive control drug. The content of Fib, and PT, APTT and TT were measured withrelated kits. Results Compared with the sham-operated group, the concentration of Fib significantly increased （P〈0.001） and PT, APTT and TT significantly shortened in the model group （1~〈0.001）; however, compared with the model group, morroniside significantly decreased Fib content （P〈0.01） and prolonged PT, APTT and TT （P〈0.05）. Conclusion Morroniside can antagonize the coagulation fi.mction in focal cerebral ischemia/reperfusion in rats.

关 键 词：莫诺苷 凝血 缺血 再灌注 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]