放射抗拒的宫颈癌细胞端粒酶活性对其放射敏感性影响的研究  

To explore the effective of telomerase activity on radiosensitivity of cervical cancer cell line tolerated to radiation

作　　者：陈敏[1] 邢丽娜[1] 

机构地区：[1]哈尔滨医科大学附属第二医院放疗科

出　　处：《实用肿瘤学杂志》2013年第1期17-20,共4页Practical Oncology Journal

基　　金：黑龙江省自然科学基金项目（D200974）

摘　　要：目的通过检测不同剂量照射宫颈癌细胞后生长特性及端粒酶逆转录酶的表达，探讨其与放射敏感性之间的关系。方法将同源的HeLa细胞分为四组，分别给予多次0Gy、2Gy、6Gy和10Gy的x射线照射，建立不同剂量的放射耐受模型，用TRAP—ELISA分别检测HeLa组、HeLa-2组、HeLa-6组和HeLa-10组端粒酶的活性，用RT—PCR检测其端粒酶hTERT的相对表达量，克隆形成实验检测其放射敏感参数d、B及SF2。结果HeLa组、HeLa-2组、HeLa-6组端粒酶活性分别是1．54±0．01、1．87-4-0．0l、2．03±0．02，各组间的差异具有统计学意义（P〈0．05）；hTERTmRNA的相对表达量分别为0．44±0．03、0．53-t-O．04、0．74±0．07，表达量明显升高且各组间的差异具有统计学意义（P〈0．05）；但HeLa-10组端粒酶的活性（1．02±0．02）及hTERTmRNA的相对表达量（0．21±0．02）都明显降低，与以上三组的端粒酶活性及hTERTmRNA的相对表达量各组的差异均具有统计学意义（P〈0．01）。四组细胞克隆形成率降低，放射敏感性依次降低。并且HeLa组、HeLa-2组、HeLa-6组各组端粒酶活性与各组细胞的放射敏感性呈负相关（r=-0．927，P〈0．01），以上三组的hTERTmRNA的相对表达量与各组细胞的放射敏感性亦呈负相关（r=-0．904，P〈0．01）。结论在-定剂量限度内，经过不同剂量的射线照射的宫颈癌HeLa细胞系，其端粒酶活性、hTERTmRNA的相对表达与其放射敏感性存在-定相关性。Objective To investigate the correlation of the telomerase activity and radiosensitivity of surviving progeny from different doses irradiated cervical carcinoma cell. Methods The HeLa cells were irradited with 0,2,6,10 Gy of X -ray for three times and the surviving progeny were subculture. There were four groups： HeLa, HeLa- 2, HeLa- 6, HeLa- 10. The telomerase activity was measured by the polymerase chain reaction - based telomeic repeat amplification protocol coupled with ELISA. And the expression of the hTERT mRNA were detected by the RT - PCR assay. The radiosesitivity index was examined by clone formation assay. Results The HeLa cells were treated by different doses of irradiation. The telomerase activity of HeLa group HeLa -2 group HeLa - 6 group was（ 1.54 ± 0.01 ）, （ 1.87 ± 0.01 ） , （2.03 ± 0.02 ） and the expression of hTERT mRNA was increased with the irradiation dose and was （ 0.44 ± 0.03 ）, （ 0.53 ± 0.04 ）, （ 0.74 ± 0. 07 ）. Each parameter had significant differences among the survival progenies （P 〈 0.05 ）. But the HeLa - 10 group was （ 1.02 ± 0. 024 ） and （0.21 ± 0. 02 ） significantly reduced. Each group＇s radiosensitivity was declined with the different irradiation dose. The telomerase activity and the expression of the hTERT mRNA was negatively correlated with radiosensitiv- ity of each groups （ r = - 0.927,P 〈 0. 01 ： r = - 0. 904, P 〈 0.01 ）. Conclusion Radiosensitivity of the surviving progeny correlates with the telomerase activity and the expression of hTERT.

关 键 词：宫颈癌 HELA细胞系 端粒酶活性 放射敏感性 端粒酶逆转录酶 

分 类 号：R737.33[医药卫生—肿瘤]