顺铂不同给药方式对鼻咽癌细胞毒性以及细胞周期影响体外研究  

In vitro study of effect of different treatment protocols of cisplatin on cytotoxicity and cell cycle in nasopharyngeal carcinoma cell line

作　　者：李丹[1] 楚建军[2] 

机构地区：[1]泸州医学院附属医院肿瘤科,四川泸州646000 [2]苏州大学附属第四医院肿瘤放疗科

出　　处：《泸州医学院学报》2013年第1期61-64,共4页Journal of Luzhou Medical College

摘　　要：目的:采用细胞培养方法研究顺铂不同给药方式对鼻咽高分化鳞癌细胞株CNE-1的细胞毒性以及细胞周期的影响。方法:MTT方法检测顺铂对鼻咽癌CNE-1细胞的半抑制浓度(IC50),1/5IC50为小剂量化疗参考剂量,而IC70则为MTD化疗参考剂量。细胞分4组进行如下处理:A组剂量为1/5IC50的顺铂连续作用96 h;B组剂量为IC70的顺铂连续作用3 h;C组为IC70作用3 h后间隔93 h;D组为未加入顺铂化疗的鼻咽癌细胞株同样条件下培养96 h。采用流式细胞仪进行细胞周期的检测。结果:顺铂对鼻咽癌CNE-1细胞株作用72 h的IC50值为0.26μg/ml,1/5IC50为0.05μg/ml,IC70为0.72μg/ml。顺铂为0.05μg/ml剂量时对CNE-1细胞无明显毒性,但细胞周期研究发现,当其连续作用96 h时,与阴性对照组相比可引起明显G2/M期阻滞(P<0.05);而顺铂剂量为0.72μg/ml连续作用3 h后间隔24 h以及93 h时检测细胞周期变化也出现显著G2/M期阻滞(P<0.05);但在0.72μg/ml的顺铂作用3 h后立即检测细胞周期变化,与相应阴性对照组相比无统计学意义(P>0.05)。结论:体外低毒性的小剂量顺铂连续给药可诱导鼻咽癌细胞株CNE-1细胞周期G2/M期阻滞。Objective：To study the effect of different treatment protocols of cisplatin on cytotoxicity and cell cycle in well-differentiated nasopharyngeal squamous cell carcinoma cell line CNE-I. Methods：MTT assay was used to evaluate the half inhibitory concentration （IC50） of cisplatin. The reference dose of low-dose chemotherapy was 1/5IC50, and IC70 as MTD reference dose. Nasopharyngeal carcinoma cells were treated with cisplatin by four treatment protocols： 96h exposure to dose of 1/5IC50 （protocol A）; 3h exposure to dose of IC70 （protocol B）; 3h exposure to IC70 followed by a 93h interval（protocol C）;cells not exposed to cisplatin were cultured under the same conditions for 96h （protocol D）.The change of cell cycle were studied by flow cytometry. Results： The IC50 of 72h exposure to cisplatin was 0.26 μg/ml , and the dose of 1/5IC50 and lCTo was 0.05 μg/ml and 0.72μg/ml, respectively. It had no significant toxicity when the dose of cisplatin was 0.05 μg/ml, however the cell cycle study found that compared with the negative control group G2/M phase was significantly arrested after 96h exposure to this dose （P〈0.05）,3h expose to 0.72 μg/ml of cisplatin with a 24h interval and 93h interval could induce significant G2/M phase arrest （P〈0.05）, whereas no cell cycle arrest was found immediately after 3h exposure to 0.72 μg/ml of cisplatin （P〉0.05）. Conclusion：The protocol of low-dose continuous administration of cisplatin, which was hypotoxic, could induce G2/M phase arrest in vitro study.

关 键 词：节律化疗 鼻咽高分化鳞癌细胞株 顺铂 细胞周期 

分 类 号：R739.62[医药卫生—肿瘤]