ARK5和基质金属蛋白酶2在小细胞肺癌中的表达及其临床意义  

Expression of ARK5 and MMP-2 in Small Cell Lung Cancer and Its Clinical Significance

作　　者：陶蓉[1] 邱世超[1] 侯成浩 李梦云[1] 宿超[1] 李慧[1] 王云甲[1] 史立宏[1] 张宝刚[1] 郑世良[3] 

机构地区：[1]潍坊医学院,山东省潍坊市261053 [2]山东省邹城市人民医院呼吸内科 [3]潍坊医学院附属医院呼吸科

出　　处：《中国全科医学》2013年第6期633-636,共4页Chinese General Practice

基　　金：国家自然科学基金(81072068);山东省中青年科学家科研奖励基金(2010BSB14050);潍坊市科学技术发展计划项目(201201230)

摘　　要：目的探讨ARK5和基质金属蛋白酶2(MMP-2)在小细胞肺癌(SCLC)组织中的表达及其临床意义。方法采用免疫组化方法检测106例SCLC癌组织(SCLC组)及40例正常肺组织(对照组)中ARK5和MMP-2的表达,分析ARK5、MMP-2的表达与患者病情分期及预后的关系。结果 SCLC组ARK5和MMP-2阳性率分别为67.9%(72/106)和65.1%(69/106),对照组中分别为5.0%(2/40)和7.5%(3/40),差异均有统计学意义(χ2值分别为46.004和38.540,P<0.05)。ARK5和MMP-2的阳性率与肿瘤直径、分化程度、浸润深度、淋巴结转移及肿瘤分期有关(P<0.05)。Spearman秩相关分析显示,SCLC癌组织中ARK5与MMP-2呈正相关(r=0.878,P<0.001)。Kaplan-Meier生存曲线显示,ARK5阳性组5年生存率为12.9%,阴性组为59.6%,差异有统计学意义(χ2=30.077,P<0.001);MMP-2阳性组5年生存率为16.3%,阴性组为62.3%,差异有统计学意义(χ2=24.405,P<0.001)。Cox多因素分析显示,ARK5、分化程度、肿瘤分期对SCLC患者预后的影响有统计学意义(P<0.05)。结论 ARK5和MMP-2的表达与SCLC的分化程度、浸润深度、淋巴结转移和肿瘤分期密切相关,它们都对SCLC患者的生存率产生重大影响,同时ARK5是SCLC患者预后的独立危险因素。Objective To investigate the expression of ARK5 and MMP- 2 in small cell lung cancer （SCLC） and its clinical significance. Methods Immunohistochemistry method was used to detect the expressions of ARK5 and MMP - 2 in 106 cases of SCLC tissues and 40 cases of normal lung tissues. The expressions of ARK5 and MMP - 2 and its relationship with pa- tients＇ condition and prognosis were analyzed. Results The positive rate of ARK5 and MMP - 2 was 67. 9% （72/106） and 65.1% （69/106） respectively in SCLC group and 5.0% （2/40） and 7.5% （3/40） respectively in control group, and the difference was statistically significant （X2 were 46. 004 and 38. 540, P 〈 0.05 ） . The positive rate of ARK5 and MMP - 2 was correlated with tumor size, differentiation degree, invasion depth, lymph node metastasis and tumor stage （P 〈 0.05 ）. Spearman correlation analysis showed that there was positive correlation between the expressions of ARK5 and MMP - 2 （ r = 0. 878, P 〈 0. 001 ） . Kaplan -Meier survival analysis showed that the five -year survival rate in patients with positive ARK5 ex- pressions was 12. 9% , significantly lower than that of patients with negative expression （χ2 = 30. 077, P 〈 0. 001 ）, while the five -year survival rate in patients with positive MMP -2 expression was 16. 3%, significantly lower than that of patients with negative expression 62. 3% （χ2 = 24. 405, P 〈 0. 001 ） . Cox multi - factor analysis showed that ARK5, differentiation degree, tumor stage were independent prognostic factors for SCLC （ P 〈 0. 05） . Conclusion The expressions of ARK5 and MMP - 2 are closely related to differentiation degree, invasion depth, and lymph node metastasis and tumor stage in SCLC patients. ARK5 and MMP -2 can greatly influence patients＇ survival rate, and meanwhile, ARK5 is an independent risk factor for the prognosis of SCLC patients.

关 键 词：肺肿瘤 基质金属蛋白酶2 免疫组织化学 肿瘤转移 预后 

分 类 号：R734.2[医药卫生—肿瘤]