肝细胞生长因子对小鼠实验性肠炎P-选择素表达的影响  

Influence of Hepatocyte Growth Factor on P-selectin in Experimental Colitis of Mice

作　　者：殷瓅[1] 陆允敏[1] 金湧[1] 陈维雄[1] 朱金水[1] 陈尼维[1] 

机构地区：[1]上海交通大学附属第六人民医院消化内科,上海200233

出　　处：《河北医学》2013年第4期503-507,共5页Hebei Medicine

摘　　要：目的:研究肝细胞生长因子(HGF)对噁唑酮诱导的小鼠实验性肠炎的P-选择素表达量的影响,并探讨其机制。方法:健康昆明小鼠60只分为6组:正常组、模型组、生理盐水(NS)组、肝细胞生长因子(HGF)组、柳氮磺胺吡啶(SASP)组、联合用药组(SASP+HGF),每组各10只。除正常组外,其余小鼠均以噁唑酮灌肠造模。造模后NS组使用NS腹腔注射(0.1mL.只-1.d-1),HGF组使用HGF腹腔注射(100ug.只-1.d-1),SASP组使用SASP灌胃(200mg.kg-1.d-1),联合用药组使用HGF腹腔注射(100ug.只-1.d-1)及SASP灌胃(200mg.kg-1.d-1)。第13 d处死所有小鼠。提取小鼠肠粘膜固有层单个核细胞(LPMC),用荧光PCR检测P-选择素表达量。结果:模型组及NS组的P-选择素表达量明显高于正常组(P<0.01)。HGF组、SASP组和联合用药组P-选择素表达量较模型组和NS组显著降低(P<0.01)。HGF组和联合用药组P-选择素表达量与正常组相比,无统计学意义。SASP组P-选择素表达量较正常略有下降(P<0.05)。但三组间无统计学差异(P>0.05)。结论:P-选择素在噁唑酮诱导的小鼠实验性肠炎中表达增强,肝细胞生长因子对P-选择素表达量有抑制作用。Objective： To investigate the influence of hepatocyte growth factor on P-selecdn in experi-mental colitis of mice, and the potential mechanism of HGF. Method： Sixty mice were randomized into 6 groups ： normal group, model group, normal saline treated group （ NS ） , HGF treated group （ HGF）, SASP treated group and HGF and SASP treated group. Except the mice in normal group, all mice were induced by oxazolone enema. NS was intraperitoneally injected in the mice of NS group（0.1mL·body-1·d-1）after enema . HGF was intraperitoneally injected in the mice of HGF group （ 100ug·body-1· d-1 ） after enema. SASP was given by garage in the mice of SASP group （200mg · kg-1 · d-1 ） after enema. The mice of HGF and SASP group were treated with both HGF and SASP. All mice were sacrificed ced at 13th day. Gene expression of P-selectin were detected by fluorescence quantitative PCR（ FQ-PCR}. Result： The expression of P-selectin mRNA in model group and NS group were higher than those in normal group（ P〈0.01）. HGF group,SASP group and HGF＆SASP group were lower than those in model group and NS group）P〈0.01）. HGF group and HGF＆SASP group were no statistical significance with normal group（ P 〉0.05）. SASP group was lower than in normal group （ P 〈 0.05 ）, There was no statistical significance between HGF group, SASP group and HGF＆SASP group （ P 〉0.05 ）. Conclusion： The results indicate that expression of P-selectin is increased in oxazolone-induced colitis and can be inhibited by HGF.

关 键 词：肝细胞生长因子 噁唑酮 P-选择素 结肠炎 

分 类 号：R574.62[医药卫生—消化系统]