降解油茶粕发酵生产芽孢杆菌培养基条件优化  

Optimizing of Culture Medium Condition for Yielding Bacillus Subtilis Using Degradation Camellia Meal

作　　者：丁丽霞[1] 黎继烈[1] 朱晓媛[1] 钟海雁[1] 

机构地区：[1]中南林业科技大学发酵工程实验室,长沙410004

出　　处：《中国食品学报》2013年第2期123-129,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家"十一五"科技支撑项目(2009BADB1B10)

摘　　要：以降解油茶粕为原料,液态发酵生产枯草芽孢杆菌。经单因素实验、Plackett-Burman实验、最陡爬坡实验确定培养基组成因素的响应值及中心点,通过正交实验优化生产枯草芽孢杆菌的培养基条件。试验结果显示:优化的培养基条件是:降解油茶粕13g,可溶性淀粉1g,尿素1.5g,磷酸氢二钠0.05g,七水硫酸镁0.015g,牛肉膏0.2g,七水硫酸亚铁0.03g,酵母膏0.5g,蒸馏水100mL,自然pH。于121℃灭菌30min后接种5mL菌悬液,37℃150r/min摇床培养24h,得到含108个/mL的枯草芽孢杆菌液态发酵液。Used degradation camellia meal as raw material producted Bacillus subtilis in liquid fermentation. The single factor experiment, Plackett-Burman experiment, the steepest climbing experimentally determined the response and the center value of culture medium composition. And then, through the orthogonal experiments eventually determined manufacturing bacillus subtilis culture medium condition is degradation camellia meal 13 g, soluble starch 1 g, urea 1.5 g, Na2HPO4 0.05 g, MgSO4·7H2O 0.015 g, beef cream 0.2 g, FeSO4·7H2O s 0.03 g, yeast anointed 0.5 g, distilled water 100 mL, natural pH, 121 ℃ sterilization 30 min, 5 mL bacteria, 37 ℃, 150 r/min wave bed and the fermentation time is 24 h, we got fermented liquid with 108/mL of Bacillus subtilis.

关 键 词：降解油茶粕 活菌数 枯草芽孢杆菌 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程]