正交函数法测定血中咖啡因含量的实验研究(英文)  

Experimental Study on Quantitative Determination of Blood Caffeine by OFS

作　　者：徐强[1] 宋学军[1] 谢妮[2] 赵金生[2] 

机构地区：[1]沈阳师范学院化学系,辽宁沈阳110034 [2]中国医科大学,辽宁沈阳110001

出　　处：《沈阳师范学院学报（自然科学版）》2000年第4期25-30,共6页Journal of Shenyang Normal University(Natural Science)

摘　　要：应用Glenn正交发光光度法测定血中咖啡因的含量 ,正交多项式为a(λ) ,波长点数为 5,波长范围 2 60nm～2 80nm ,波长间隔△λ=5nm ,中心波长λm=2 72nm ,不相关吸收被减小到可忽略不计 ,正交多项式 (P2 )与不同浓度的咖啡因之间的线性关系良好 (r=0 .9999) .方法的实验平均回收率为 99.8% ,变异系数 0 .3 9% .Glenn's orthogonal function spectrophotometry has been applied to quantitative determination of blood caffeine. With polynomial  2(λ), number of points, 5, wavelength range 260-280nm, interval △λ=5nm, and mean wavelength λ m=272nm, the irrelevant absoroption was reduced to a negligible value, and a satisfactory linear relation between the orthogonal polynomial (p2) and the concentration of caffeine was obtained (r=0.9999). The mean recovery rate is 99.8% and CV is 0.39%. The results showed that this method is suitable for routine forensic chemical analysis.

关 键 词：正交函数 分光光度法 咖啡因 含量测定 血液 

分 类 号：R446.11[医药卫生—诊断学]