鲨鱼软骨血管生成抑制因子的抗体制备与组织特异性表达的检测  

Preparation of Antibody of Shark Cartilage-derived Angiogenesis Inhibitory Factor(SCAIF-Ⅰ) and Examination of Its Special Expression in Different Tissues

作　　者：胡幼卿[1] 沈先荣[1] 邓新贤[1] 吉冬梅[1] 胡忻然[1] 黄建生[1] 任大明[1] 

机构地区：[1]复旦大学生命科学学院遗传工程国家重点实验室,上海200433

出　　处：《复旦学报（自然科学版）》2000年第5期560-563,共4页Journal of Fudan University：Natural Science

摘　　要：纯化鉴定了鲨鱼软骨血管生成抑制因子 (SharkCartilage derivedAngiogenesisInhibitoryFactor Ⅰ ,SCAIF Ⅰ )的纯度及分子质量 .采用新西兰大白兔背部皮下多点注射法制备了SCAIF Ⅰ的多克隆抗体 ,测定了抗体的效价 .通过Westernblot检测 ,证明了SCAIF Ⅰ相关蛋白在哺乳动物中的广泛分布及SCAIF Ⅰ在鲨鱼软骨中的组织特异性表达 .The purity and relative molecular mass of SCAIF Ⅰ have been determined. Its multiclonal antibody was produced in Newzealand rabbits through hypodermic injection on the back. By Western blotting, it was proved that the SCAIF Ⅰ related protein is widely distributed in mammal, and expression of SCAIF Ⅰ is tissue specific, only in shark cartilage.

关 键 词：鲨鱼 软骨 血管生成 抑制因子 抗体制备 组织 

分 类 号：Q959.42[生物学—动物学]