基于线粒体D-loop基因序列研究我国5种虾虎鱼类的系统进化关系  

Phylogenetic relationships of 5 gobioidei species based on sequences of mitochondrial DNA D-loop

作　　者：侯新远[1] 祝斐[1,2] 张丽娟[1] 尹绍武[1] 胡亚丽[1] 贾一何[1] 李丽[1] 汪亚媛[1] 张国松[1] 

机构地区：[1]南京师范大学生命科学学院,江苏省生物多样性与生物技术重点实验室,南京210023 [2]海南大学海洋学院,海口570228

出　　处：《海洋渔业》2013年第1期1-7,共7页Marine Fisheries

基　　金：国家星火计划项目(2011GA690368);江苏省农业科技自主创新资金项目[CX(11)1037];江苏省农业科技支撑计划项目(BE2011331);南京师范大学高层次人才科研启动基金项目(2011104XGQ0071);江苏高校优势学科建设工程资助项目

摘　　要：利用PCR技术扩增河川沙塘鳢(Odontobutis potamophila)、鸭绿沙塘鳢(Odontobutis yaluensis)、中华沙塘鳢(Odontobutis sinensis)、葛氏鲈塘鳢(Perccottus glenii)及尖头塘鳢(Eleotris oxycephala)的线粒体D-loop区并测序,获得河川沙塘鳢、鸭绿沙塘鳢、中华沙塘鳢、葛氏鲈塘鳢及尖头塘鳢线粒体D-loop区全序列分别为853bp、853bp、1028bp、852bp、848bp,结合GenBank中平头沙塘鳢(Odontobutis platycephala)、刺盖塘鳢(Eleotris acanthopoma)、黑体塘鳢(Eleotris melanosoma)、褐塘鳢(Eleotris fusca)、中华乌塘鳢(Bostrychussinensis)、云斑尖塘鳢(Oxyeleotris marmorata)、溪鳢(Rhyacichthys aspro))等7种虾虎鱼类同源的D-loop区序列,分析了这12种虾虎鱼类的系统发育关系。所使用的同源序列长度为832～846bp,其中保守位点452个,变异位点405个,简约信息位点258个,单态位点145个,转换/颠换平均值为0.8。基于Kimura2-parameter模型的12种虾虎鱼类遗传距离范围为0.043～0.312,在Kimura2-parameter模型构建的邻接树和非加权配对法系统树中,河川沙塘鳢、鸭绿沙塘鳢、中华沙塘鳢、平头沙塘鳢聚为一大支,刺盖塘鳢、黑体塘鳢、褐塘鳢、尖头塘鳢、溪鳢、葛氏鲈塘鳢聚为另一支。To investigate the molecular phylogeny relationship of Gobioidei species fish, D-loop of five speciesfish （Odontobutis potamophila, Odontobutis yaluensis, Odontobutis sinensis, Perccottus glenii and Eleotris oxycephala） were cloned by PCR methods. PCR amplification products of complete D-loop gene fragments from five species fish were 853 bp , 853 bp , 1 028 bp , 852 bp and 848 bp , respectivelyl Together with homologous D-loop sequences of seven species （Odontobutis platycephala, Eleotris acanthopoma, Eleotris melanosoma, Eleotris fusca , Bostrychus sinensis , Oxyeleotris marmorata and Rhyacichthys aspro ）from GenBank, D-loop sequences of twelve species fish were analyzed by MEGA 4.0 software. The results showed that mitochondrial DNA D-loop sequences were 832-846 bp , of which 452 sites were conserved, 405 sites were variable, 258 sites were parsimony informative, 145 sites were singleton sites and the average transitionsal/transversional was 0. 8. Kimura 2-parameter model-based pairwise genetic distances within twelve Gobioidei species were analyzed, and each putative species ranged from 0. 043 -0. 312. And based on phylogenetic trees （N J） and （UPGMA）analysis, the results showed that O. potamophila, 0. yaluensis , 0. sinensis and 0. platycephala belonged to the same branch, E. acanthopoma , E. melanosoma, E. fusca , E. oxycephala , R. aspro and P. glenii belonged to another branch.

关 键 词：虾虎鱼类 D—loop 系统进化 

分 类 号：Q731[生物学—分子生物学]