MiR-451在肺癌细胞A549及肺癌癌组织中的表达及其意义  

Expression of miR-451 in lung carcinoma and its significance

作　　者：张继琛[1,2] 娄凯[1,2] 闫李侠[1,2] 

机构地区：[1]浙江省台州市第一人民医院 [2]温州医学院附属黄岩医院,浙江台州318020

出　　处：《吉林医学》2013年第4期605-607,共3页Jilin Medical Journal

摘　　要：目的:观察微小RNA(miRNA)mir-451在肺癌组织、肺癌细胞株A549中的表达,同时检测该miRNA在正常肺组织及肺细胞癌组织中的表达,探讨其在肺癌发生及发展中的作用和意义。方法:应用实时定量聚合酶链反应(PCR)方法检测mir-451在正常细胞株及肺癌细胞株中的表达,同时收取肺细胞癌手术患者正常组织、癌周组织、癌组织,检测其表达并分析其意义。结果:mir-541在肺癌细胞(A549)中的表达比正常肺细胞显著降低,分别为0.294±0.066和0.894±0.023,两者比较差异有统计学意义(P<0.05),在癌组织中表达显著低于正常组织与癌周组织,分别为0.124±0.002、0.870±0.034、0.896±0.008,差异有统计学意义(P<0.05),正常组织及癌周组织比较差异无统计学意义(P>0.05)。结论:mir-145在肺癌细胞及肺癌组织中的低表达,可能与肺癌发生和发展密切相关,并可能作为肺癌的诊断及预后的指标。Objective To investigate the expression of miR -451 in human lung carcinoma A549, lung carcinoma tissues and normal lung,and explore the role of miR -451 in human lung carcinoma. Method We detected the expressions of miR -451 in normal cell and lung carcinoma cell, and also detected the expressions of normal tissue,peritumoral tissue, cancer tissue of lung cell carcinoma surgery pa- tients by real- time PCR, and analyze the importance. Results The expression of miR- 451 in human lung carcinoma A.549 was signifi- candy lower than the human normal lung （0. 294 ± 0. 066,0. 894 ± 0. 023, P 〈 0. 05）, and compared with the cancer orgalfizations and nor- mal lung, the expression of miR -451 in human lung carcinoma tissues decreased significantly （0. 124 ± 0. 002,0. 870 ± 0. 034,0. 896 ±0. 008, P 〈 0. 05 ）. Conclusion These results indicate that the under -expression of miR -541 is probably related to human lung carcinoma pathogenesis and it can be the indicator of diagnosis and prognosis of the lung carcinoma.

关 键 词：miR-451 肺细胞癌 实时定量PCR 

分 类 号：R734.2[医药卫生—肿瘤]