阿的平对高功率微波辐射小鼠肺损伤的保护作用  

Protective effects of quinacrine on lung injury induced by microwave irradiation in mice

作　　者：吴燕[1] 刘淑红[1] 林凯[2] 葛学铭[1] 王飞[1] 苏振涛[3] 周红梅[3] 赵永岐[1] 范明[1] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850 [2]空军总医院临床检验中心,北京100142 [3]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《感染．炎症．修复》2012年第4期212-214,F0002,共4页Infection Inflammation Repair

基　　金：国家自然科学基金资助项目(31071042);军队医药科研"十五"专项课题(01L024)

摘　　要：目的:探讨阿的平对高功率微波辐射小鼠肺组织的保护作用。方法:130只BALB/c小鼠随机分为4组:正常组、辐射对照组、阿的平低剂量组(12.6 mg/kg)、阿的平高剂量组(50.4 mg/kg)。辐射对照组小鼠灌胃给予注射用水20 ml/kg,两阿的平组分别灌胃给予不同剂量阿的平,1 h后用50 mW/cm^2的微波照射30 min。正常组于实验7 d,其余3组于照射后即刻、1 d、2 d、7 d处死小鼠取肺脏,HE染色,观察肺组织形态学变化;Westernblot检测肺组织中热休克蛋白70(HSP70)表达的变化。结果:50 mW/cm^2微波照射30 min后,辐射对照组出现肺组织内瘀血现象,肺泡上皮细胞脱落,肺泡间隔变薄、断裂,支气管充血,直至照射后7 d。阿的平给药组肺间隔断裂减少,组织淤血现象减轻,照射后2 d组织形态的改善最为显著,尤以高剂量组保护作用更明显。从微波照射后即刻,辐射对照组和阿的平组的小鼠肺中HSP70表达开始升高,直至7 d。阿的平给药组与辐射对照组相比HSP70表达明显上调,尤以阿的平高剂量组上调明显,但两阿的平给药组之间差异无显著性(P>0.05)。结论:阿的平能上调高功率微波辐射后小鼠的HSP70蛋白表达水平,减轻辐射后小鼠肺组织的病理损伤,对微波辐射小鼠的肺组织有一定的保护作用。Objective：To investigate the effect of quinacrine on lung injury induced by microwave irradiation in mice. Methods：One hundred and thirty BALB/c mice were randomly divided into four groups., normal control group （n=10）, radiation control group （n=40）, quinacrine low-dose group （12.6 mg/kg, n=40）and quinacrine high-dose group （50.4 mg/kg, n=40）. Mice of the two quinacrine groups received quinacrine by gavage （20 ml/ kg） 1 hour before microwave irradiation, and normal saline was given instead of quinacrine in the radiation control group. All animals, except those of normal control group, received 50 mW/cm2 microwave irradiation for 30 mi-nutes. Pathological changes in lung were observed immediately after irradiation, and 1 day, 2 days, 7 days after irradiation in radiation control group and quinacrine groups, and 7 days after irradiation experiment in normal con-trol group. Meanwhile, heat shock protein 70 （HSP70）protein expression in lung was analyzed using Western Blot. Results：Pathological examination showed that 50 mW/cm^2 of microwave irradiation for 30 minutes induced lung damages as manifested by blood stasis, alveolar epithelial cell shedding, alveolar septal thinning and fracture, and bronchial congestion in mice lungs. However, in quinacrine groups, reduction in lung septal fracture and at-tenuation of lung tissue congestion were found compared with radiation control group. The improvement were more markedly at 2 days after irradiation, especially in quinacrine high dose group. Microwave irradiation up-regu-lated the expression of HSP70 to certain degree in lung, while quinacrine pretreatment further up-regulated the ex-pression of HSP70, especially in quinacrine high dose group. Conclusions：The mechanism of quinacrine protection on lung injury induced by microwave irradiation may be attributed to an increase of HSP70 expression.

关 键 词：阿的平 微波 辐射 肺损伤 

分 类 号：R57[医药卫生—消化系统]