可溶性Fas表达水平与人黑色素瘤相关性的初步研究  

Expression of soluble Fas is correlated to human malignant melanoma

作　　者：谭岩[1] 王永晨[2] 白晓[3] 芦小单[1] 方艳秋[1] 

机构地区：[1]吉林省人民医院医学诊治实验中心,长春130021 [2]哈尔滨医科大学第一临床医院,哈尔滨150001 [3]吉林大学医学院2007届七年制,长春130000

出　　处：《中国免疫学杂志》2013年第2期154-157,共4页Chinese Journal of Immunology

基　　金：吉林省科技厅基础研究项目(201015216);吉林省科技厅重点实验室项目(20122113);吉林省科技厅科技创新项目(20082102);黑龙江省卫生厅资助课题(2007-247)资助

摘　　要：目的:分析可溶性Fas(soluble Fas,sFas)表达水平与人黑色素细胞瘤恶性程度的关系,探讨Fas、Fas配体与可溶性Fas在调控细胞凋亡信号转导途径中的作用。方法:对35位确诊黑色素细胞癌患者的皮肤组织标本和病例进行回顾性分析和归类,用免疫荧光的方法检测组织中Fas/FasL的表达水平。通过酶联免疫吸附反应(ELISA)方法对人血清中sFas的浓度进行分析。结果:35例黑色素细胞癌组织中,病理分型浅表型2例、结节型9例、肢端型18例、雀斑痣型6例。Fas在正常皮肤组织中高表达,恶性黑色素癌组织中低表达;而相反FasL在恶性黑色素癌组织中高表达,在正常皮肤组织中表达非常低。可溶性Fas在不同组织中的表达强度差异显著,sFas在恶性黑色素细胞癌患者、良性黑色素瘤和正常人血清中的浓度分别为(36.32±11.57)ng/ml、(7.33±3.78)ng/ml和(4.18±1.88)ng/ml。结论:可溶性Fas在恶性黑色素癌患者血清中含量较高,与黑色素细胞癌恶性程度呈正相关,sFas对细胞凋亡的竞争性抑制可能与黑色素细胞癌的进展密切相关。Objective ：To investigate the apoptosis related mechanisms of Fas/Fas ligand and soluble Fas in malignant mela- nomaby by analyze soluble Fas（sFas） expression in primary human malignant melanoma. Methods：Analyze the pathological and dis- ease staging records by reviewing 35 cases of human malignant melanoma, determine Fas/FasL expression by immunoflourescence. An- alyze soluble Fas expression levels in serum samples of patients by ELISA. Results：In 35 cases of malignant melanoma, there were 2 cases of superficial spreading melanoma, 9 cases of nodular melanoma, 18 cases of acral letiginous melanoma and 6 cases of lentigo maligna melanoma. Fas expression was high in normal skin tissues and low in malignant melanomas, when FasL expression was the o19_ posite. The soluble Fas levels showed significant differences in malignant melanoma, compare to melanocytic nevus and normal people were （ 36.32 ± 11.57 ） ng/ml, （7.33 ± 3.78 ） ng/ml and （4.18 ± 1.88 ） ng/ml respectively. Conclusion ： Soluble Fas was highly ex- pressed in malignant melanoma, which is correlated to the malignancy. Soluble Fas may competitively play important roles in apoptosis mechanisms of malignant melanoma.

关 键 词：细胞凋亡 黑色素细胞瘤 可溶性FAS FAS FAS配体 

分 类 号：R733[医药卫生—肿瘤]