原位杂交技术检测菲律宾蛤仔的帕金虫病  

Detecting perkinsus.sp in the Manila clam Ruditapes philip pinarum by in Situ Hybridization

作　　者：王丽[1,2] 梁斌[3] 卢亚楠[1] 丛玉婷[1] 刘丹[1] 梁玉波[2] 

机构地区：[1]农业部北方海水增养殖重点实验室大连海洋大学,辽宁大连116023 [2]辽宁省水生生物学重点实验室大连海洋大学,辽宁大连116023 [3]国家海洋环境监测中心,辽宁大连116021

出　　处：《河北渔业》2013年第3期17-19,29,共4页

摘　　要：根据帕金虫基因的特点及它的靶基因序列,从已构建的帕金虫基因组文库中,设计合成了核酸探针序列。采用原位杂交的方法,对菲律宾蛤仔体内的一种帕金虫进行原位杂交检测。实验结果表明,该方法可以从患病的菲律宾蛤仔的切片中检测到该寄生虫,对该寄生虫的mRNA均有阳性杂交信号出现。Perkinsus. sp DNA fragment from the perkinsus, sp genormic library constituted by us was used as prodes to detect perkinsus in situ hybridization. The genus-Perkinsus DNA prode was de signed to specifically target SSUrRNA gene sequences. Results indicate： a tissue section containing parasite was detected Perkinsus. sp in ill clam by ISH. The mRNA of Perking＇us. sp were positive.

关 键 词：帕金虫 原位杂交 菲律宾蛤仔 

分 类 号：S944[农业科学—水产养殖]