TAT PTD-Tachyplesin融合基因的重叠延伸PCR法合成  

The Artificial Synthesis of TAT PTD and Tachyplesin Fusion Gene by Overlap Extension PCR

作　　者：邱道寿[1] 刘晓津[1] 王军[2] 苏永全[2] 

机构地区：[1]广东省农业科学院作物研究所广东省农作物遗传改良重点实验室,广东广州510640 [2]厦门大学海洋与地球学院,福建厦门361005

出　　处：《安徽农业科学》2013年第4期1438-1441,共4页Journal of Anhui Agricultural Sciences

基　　金：广东省自然科学基金(06025389);福建省博士后项目

摘　　要：为探讨HIV-Tat蛋白转导域(protein transduction domain,PTD)对中国鲎抗菌肽tachyplesin的协同作用,试验以经毕赤酵母密码子优化后的tachyplesin成熟肽(54 aa)加TAT PTD(11 aa)的cDNA序列为参考模板,设计了6条单链寡核苷酸片段,首尾引物的5'端分别引入EcoR I和Xba I酶切位点,通过重叠延伸PCR方法成功合成了TAT PTD+Tachyplesin序列,序列全长219 bp,为其后续功能与协同作用研究奠定了基础。In order to study the coeilicient ot the TAT PTD （the protein transductlon domain m HIV-I transactlvator ot transcnption protein） to the antibacterial peptide tachyplesin from Tachpleus tridentatus, 6 sequence of oligonucleotides with EeoR Iand Xba I in the ends of 5＇-end of the first and last primers respectively were designed, based on the TAT PTD and taehyplesin mature peptide cDNA template, which was op- timized according to the Piehia pastoris preferred codon. A optimized TAT PTD and tachyplesin fusion gene with 219 base pairs was artificially synthesized by overlap extension PCR, which laid a preliminary foundation for the following functional and coefficient studies.

关 键 词：PTD TAT 鲎素 重叠延伸PCR 

分 类 号：S188[农业科学—农业基础科学]