柳叶蜡梅叶挥发油的提取工艺及对脂自由基的清除作用  

Extraction Technology of Volatile Oil from Leaf of Chimonanthus Salicifolicus Hu and Scavenging Capacity against Alkyl Radical

作　　者：韦梦鑫[1] 刘智慧[1] 查钱慧[1] 张维维[1] 阮东[1] 毕淑峰[1] 

机构地区：[1]黄山学院生命与环境科学学院,安徽黄山245041

出　　处：《安徽农业科学》2013年第4期1724-1724,1747,共2页Journal of Anhui Agricultural Sciences

基　　金：黄山学院博士启动基金项目(2012xkjq002);黄山学院大学生创新基金项目(2012xdkj004)

摘　　要：[目的]优化超声波辅助法提取柳叶蜡梅叶挥发油的工艺,并研究柳叶蜡梅叶挥发油对脂自由基的清除作用。[方法]以超声时间、提取时间、料液比3个因素进行正交试验,获得柳叶蜡梅叶挥发油的最佳提取工艺;以25%挥发油为原料、1.00 mg/ml维生素C为阳性对照研究挥发油对脂自由基的清除作用。[结果]柳叶蜡梅叶挥发油提取最佳工艺条件为超声时间2 h、提取时间8 h、料液比1∶9g/ml,得率达1.67%。挥发油对脂自由基清除作用的IC50值为208.09μl。[结论]柳叶蜡梅叶挥发油含量丰富,具有较好的脂自由基清除能力,值得进一步开发利用。[ Objective] The ultrasonic-assisted extraction technology of volatile oil from leaf of Chimonanthus Salicifolicus Hu was investiga- ted, and the scavenging capacity against alkyl radical of volatile oil was studied. [ Method] The orthogonal experiments were carried out to study extraction technology of volatile oil with ultrasonic time, extraction time, and material-to-liquid ratio. The scavenging capacity against al- kyl radical of 25% volatile oil was studied with 1.00 mg/ml Vc as the positive control. [ Result] The optimal extraction technology was ob- tained as ultrasonic time of 2 h, extraction time of 8 h, and material-to-liquid ratio of 1：9 g/ml. Under the optimal conditions, the extraction yield of volatile oil was determined to be 1.67%. The IC5o value of scavenging capacity against alkyl radical was 208.09 μl. [ Conclusion] The volatile oil from leaf of Chimonanthus Salicifolicus Hu was rich, and its scavenging capacity against alkyl radical was good. So, it is worth to develop for application.

关 键 词：柳叶蜡梅 挥发油 提取工艺 脂自由基 

分 类 号：S685.99[农业科学—观赏园艺]