槲皮素对人肝癌细胞生长、迁移能力的影响及机制研究  

Study on influence of quercetin on proliferation and migration of HepG2 cells and its mechanism

作　　者：周进[1] 方丽[2] 姚文秀[1] 熊竹娟[1] 周详[1] 周行[1] 谢华[1] 

机构地区：[1]四川省肿瘤医院肿瘤内科,成都610041 [2]成都医学院第一附属医院消化内科,成都610500

出　　处：《重庆医学》2013年第8期845-847,共3页Chongqing medicine

基　　金：四川省卫生厅科学基金资助项目(100572)

摘　　要：目的探讨槲皮素对人肝癌细胞(HepG2)生长增殖及迁移能力的影响及机制。方法培养对数生长的HepG2细胞,MTT试验分析槲皮素对HepG2生长的影响,划痕实验观察加入槲皮素前、后HepG2迁徙能力的变化。Western blot检测其中细胞迁移黏附因子(IQGAP1)表达的变化。结果加入槲皮素后,HepG2细胞逐渐变圆、脱落,增殖变慢;MTT实验显示HepG2细胞的生长抑制效应与槲皮素有浓度、时间依存关系;Western blot显示加入槲皮素后HepG2细胞中IQGAP1表达明显降低。结论槲皮素能明显抑制HepG2细胞生长增殖,减弱其迁移能力,从而达到抑制恶性细胞增殖转移的作用,这种能力可能是通过抑制IQGAP1表达实现的。Objective To investigate the effects of quercetin on proliferation and migraton of HepG2 cells and its mechanism. Methods HepG2 cells in logarithmic growth phase were cultured. The influence of quercetin on the growth of HepG2 cells was an- alyzed by MTT test. The cell migration change after adding quercetin was observed by the scratch test. Western blot assay was a- dopted to detect the expression change of Ras GTPase-activating-like protein（IQGAP1） in HepG2 ceils after quercetin treatment. Results After adding quercetin for 24 h or more time, HepG2 cells turned to circle, fell down from wall,and proliferated slowly. The MTT test showed that quercetin inhibited HepG2 cells_ proliferation in a time- and dose-dependent manner. Western blot showed that after adding quercetin,the expression of IQGAP1 in HepG2 cells was obviously decreased. Conclusion Quercetin can obviously inhibit the proliferation and migration of HepG2 cells to achieve to inhibit the proliferation and transferring of malignant cells. This ability of quercetin is partly realized through the inhibition of IQGAP1 gene expression.

关 键 词：肝肿瘤 实验性 槲皮素 人肝癌细胞 细胞运动 

分 类 号：R735.7[医药卫生—肿瘤]