孕鼠补充牛磺酸减少胎儿生长受限胎鼠脑细胞凋亡  

Antenatal taurine supplementation reduces cerebral cell apoptosis in fetal rats with growth restriction

作　　者：王晓凤[1] 滕慧云[2] 刘敬[3] 杨娜[3] 任晓暾[3] 

机构地区：[1]安徽医科大学北京军区总医院临床学院,合肥230032 [2]解放军第二炮兵总医院儿科 [3]北京军区总医院附属八一儿童医院新生儿重症监护中心

出　　处：《中华围产医学杂志》2013年第3期165-169,共5页Chinese Journal of Perinatal Medicine

基　　金：国家自然科学基金

摘　　要：目的通过建立胎儿生长受限（fetalgrowthrestriction，FGR）大鼠模型，探讨孕鼠补充牛磺酸对FGR胎鼠脑细胞凋亡以及胶质细胞源性神经营养因子（glialcellline—derivedneurotrophicfactor，GDNF）和半胱氨酸蛋白酶-3（caspase-3）表达的影响。方法将15只Sprague—Dawley（SD）孕鼠随机分为对照组、FGR模型组（模型组）、FGR＋孕鼠补充牛磺酸组（牛磺酸组），每组5只。通过低蛋白饮食法建立FGR模型，牛磺酸组自妊娠第12天开始于饲料中添加牛磺酸300mg／（kg·d）直至自然分娩。对照组每窝随机取2只适于胎龄胎鼠，牛磺酸组与模型组每窝随机取2只FGR胎鼠，应用原位末端脱氧核苷酸转移酶标记（terminaldeOxynucleotidyltransferasemediatednickendlabeling，TUNEL）法检测各组胎鼠脑神经细胞凋亡情况。用免疫组织化学方法检测GDNF和caspase-3表达情况。统计学方法采用单因素方差分析、Kruskal—Wallis秩和检验、SNK检验和Tamhane’S检验。结果（1）对照组、模型组及牛磺酸组胎鼠总数分别为65、60和59只，胎鼠平均体重为（6．36士0．44）、（4．55±0．45）和（5．11±0．67）g。模型组胎鼠均发生FGR，牛磺酸组胎鼠FGR发生率为76．3％（45／59），成功建立FGR模型。（2）对照组胎鼠脑组织内偶见TUNEL阳性细胞；模型组明显增多，在大脑皮质、海马、白质区均有分布；牛磺酸组较模型组则明显减少。3组TUNEL阳性细胞数分别为（0．46±0．11）、（14．76±3．42）和（6．78±1．93）个（H=429．80，P=0．000）。（3）对照组胎鼠大脑皮质内仅有少量GDNF阳性细胞分布，模型组明显增多，牛磺酸组则进一步增多。3组GDNF阳性细胞数分别为（93．56±6．73）、（120．36±6．23）和（139．56±5．28）个（H=715．17，P=0．000）。（4）对照组胎鼠大脑皮质内仅见少数caspase3阳性细胞分布，模型组明显增多，牛磺酸组较模型组显�Objective To explore the effect of antenatal taurine supplementation on cerebral apoptosis and the expression of glial cell line-derived neurotrophic factor （GDNF） and caspase-3 in fetal rats with fetal growth restriction （FGR）. Methods Fifteen pregnant Sprague-Dawley rats were randomly divided into three groups： control group, FGR model group （model group） and FGR with antenatal taurine supplementation group （taurine group）. Taurine was added into the diet of taurine group at a dose of 300 mg/（kg · d） from the 12th day of gestation until natural delivery. Two appropriate for gestational age （AGA） newborn rats were randomly selected from each mother in control group and two FGR fetal rats were randomly selected from each mother both in model and taurine groups. Apoptosis of neural cells in the brain was detected by terminal deoxynucleotidyl transferase mediated nick end labeling （TUNEL）. Changes in protein expression of GDNF and caspase-3 were detected by immunohistochemistry. Levene method,one-way ANOVA and SNK-test, or Kruskal-Wallis rank sum test and Tamhane＇ s test were applied for statistical analysis. Results （1）The total amounts of fetal rats in control group, model group and taurine group were 65, 60 and 59. The mean body weight of fetal rats were （6.36±0.44） g, （4.55 ± 0.45） g and （5.11±0.67） g, respectively. All fetal rats developed FGR in model group, while 76.3%（45/59） of fetal rats were FGR in taurine group. Therefore, FGR model was successfully established. （2） In control group, there were few expression of TUNEL positive cells in cerebral cortex. A large amount of TUNEL positive cells were found in the cortex, hippocampal and white matter area in model group, but less positive cells were identified in taurine group than in model group. The amount of apoptotic brain cells in the three groups were （0.46± 0.11）, （14.76 ± 3.42） and （6.78 ± 1.93）, respectively （H=429.80, P=0. 000）. （3）There were only small amount of GDNF

关 键 词：胎儿生长迟缓 牛磺酸 细胞凋亡 胶质细胞源性神经营养因子 半胱氨酸天冬氨酸蛋白酶3 

分 类 号：R715.3[医药卫生—妇产科学]