芸香苷对人晶体上皮细胞氧化损伤和凋亡保护作用的初步研究  

Rutoside protecting human lens epithelial cells against oxidative injury and apoptosis

作　　者：喻亚梅[1] 周艳峰[1] 

机构地区：[1]安徽医科大学第一附属医院眼科,合肥230022

出　　处：《安徽医科大学学报》2013年第4期379-382,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省卫生厅中医药科研计划课题(编号:2012zy50)

摘　　要：目的研究黄酮类化合物芸香苷(Ru)对过氧化氢(H2O2)诱导的人晶体上皮细胞(HLEC)氧化损伤和凋亡的保护作用。方法用不同浓度Ru(0、25、50、100μmol/L)预处理HLEC 1 h,再加入200μmol/L H2O2,分别检测各组细胞增殖活性以及超氧化物歧化酶(SOD)、还原型谷胱甘肽(GSH)、丙二醛(MDA)水平,观察细胞凋亡形态并计算凋亡指数(AI)。结果 200μmol/L H2O2组HLEC增殖能力及SOD、GSH含量较对照组显著下降,MDA和AI较对照组明显增高(P<0.01);Ru预处理组与H2O2组相比,细胞生存率、抗氧化水平明显提高,凋亡细胞数量显著减少(P<0.01)。结论 Ru通过提高细胞增殖能力保护H2O2引起HLEC的氧化损伤和凋亡。Objective To investigate the protective effect of flavonoid compound rutoside on hydrogen peroxide-in- duced oxidative injury and apoptosis in human lens epithelial cells （HLEC）. Methods Normal HLEC was pretrea- ted with different concentrations of rutoside （0,25,50,100 p^mol/L） for 1 h, followed by treatment with 200 p, mol/ L H2 02. The cell proliferative activity and the level of superoxide dismutase （SOD）, glutathione （GSH） and ma- londialdehyde （MDA） were measured. The cell apoptosis morphology and apoptosis index （AI） were detected. Results The proliferation ability of HLEC and the content of SOD, GSH treated with by 200 p.moL/L H20~ were significantly lower than that in control group, while MDA and AI increased evidently（ P 〈 0.01 ） ; Compared with H： O： group, the cell viability and antioxidant levels in pretreated group increased obviously, and the apoptosis cell quantity diminished significantly （P 〈 0. 01 ）. Conclusion Rutoside protects peroxide-induced oxidative injury and apoptosis in human lens epithelial cells through enhancing the cell proliferation ability.

关 键 词：晶体上皮细胞 芸香苷 过氧化氢 氧化损伤 细胞 凋亡 

分 类 号：R776.1[医药卫生—眼科]