苎麻胚珠的酶解法提取及其整体染色透明技术  

Enzymatic Isolation and Whole Stain-Clearing Techniques of Ramie Ovule

作　　者：胡慧[1] 臧巩固[1] 赵立宁[1] 李育君[1] 唐蜻[1] 程超华[1] 高春生[1] 

机构地区：[1]中国农业科学院麻类研究所,湖南长沙410205

出　　处：《中国麻业科学》2013年第1期9-13,37,共6页Plant Fiber Sciences in China

摘　　要：本文以悬铃叶苎麻雌花为试验材料,研究通过酶解方法分离出胚珠并对其进行整体染色透明的技术,目的在于提高整体透明技术在苎麻胚胎发育观察中的效果。结果表明:3%纤维素酶与1%果胶酶混合酶液提取胚珠的效果最好,采用苏木精染色后冬青油透明的方法观察效果较好。In order to observe the embryonic growth in Boehmeria tricuspids better, the ovules were isolated from the female flowers by enzymatic method and the corresponding whole stain - cleating technique was studied. Results showed that a mixture solution of 3 % cellulase and 1% pectinase had the best dissociation effect, and staining with hematoxylin, clearing with methyl salieylate were suitable for observing the ovules.

关 键 词：悬铃叶苎麻 胚珠 酶解法 整体染色透明 

分 类 号：S563.1[农业科学—作物学]