胃癌nm23基因mRNA原位杂交和免疫组化研究  

In situ hybridization and immunohistochemical analysis of expression of nm23-H_1mRNA and its productions in human gastric carcinomas

作　　者：姜惠峰[1] 姜颖君[1] 吴荣[2] 王振[1] 孙桂青 丁守怡[2] 

机构地区：[1]山东省烟台市莱阳中心医院,莱阳市265200 [2]山东省青岛大学医学院,青岛市266021

出　　处：《肿瘤防治杂志》2000年第1期21-25,共5页China Journal of Cancer Prevention and Treatment

基　　金：山东省科委科技攻关项目!(961165308C)

摘　　要：目的 :探讨胃癌mn2 3 H1基因表达与淋巴结转移倾向的相关性。方法 :采用地高辛标记cD NAnm2 3 H1探针 ,原位杂交检测 2 1例胃癌及癌旁组织 ;应用单克隆抗体 (NM30 1)SP法检测 131例胃癌及 60例非胃癌胃粘膜nm2 3/NDPK表达。结果 :原位杂交阳性率 57.1% ( 12 /2 1) ;免疫组化阳性率为87.8% ( 115/131) ,其中非转移组阳性率为 97.1% ( 34 /35) ,转移组阳性率 84 .4 % ( 81/96) ,P <0 .0 5。结论 :胃癌nm2 3 H1基因表达与淋巴结转移倾向呈负相关 。Objective:To elucidate the relationship between expression of nm23 gene in human gastric carcinomas and lymph node metastatic potential.Methods:Twentyone gastric carcinoma tissues and correponding noncancer mucosae were examined by in situ hybridization with digoxigeninlabeled nm23H 1cDNA probes.Immunohistochemical staining was made by the StreptoavidinBiotin method(SP) with mAb nm23/NDPK,131 gastric carcinoma tissues and 60 specimens of noncancer gastric mucosae were examined.Results:Twelve(571%)out of 21 gastric carcinomas expressed higher levels of nm23mRNA than corresponding noncancer mucosae.One hundred fifteen(87.8%)out of 131 gastric carcinomas exhibited possitive immunoreactivity for nm23/NDPK,97.1%(34/35)and 84.4%(81/96)were immunoreactivity for nonmetastatic groups or metastatic groups,respectively.Statistics analysis was made using the χ2 test,the P value was less than 0.05.Conclusions:Expression of nm23/NDPK in gastric carcinomas was inversely associated with lymph node metastatic potential,directively with PCNA labeling index.

关 键 词：胃肿瘤 NM23-H1 mRNA原位杂交 PCNA 

分 类 号：R735.2[医药卫生—肿瘤]