以Bio-oss胶原为支架材料的组织工程化骨修复种植体周围骨缺损的初步实验研究  

Preliminary experimental study of repairing bone defects around dental implants with tissue engineering bone established with Bio-oss collagen scaffold

作　　者：赵健[1] 黄远亮[2] 刘阿贵[1] 蒋欣泉[3] 

机构地区：[1]无锡市第四人民医院口腔科,无锡214062 [2]上海同济大学附属东方医院口腔科,上海200120 [3]上海交通大学附属第九人民医院口腔医学院口腔生物工程实验室,上海200011

出　　处：《口腔医学》2013年第2期95-99,共5页Stomatology

基　　金：无锡市卫生局指令性课题(XM0804)

摘　　要：目的研究以Bio-oss胶原为支架材料的组织工程化骨修复种植体周围骨缺损的效果。方法取4只小型猪设立自身对照,获取骨髓基质细胞(BMSCs),经成骨诱导培养液体外培养、扩增、诱导后进行I型胶原、骨钙素的免疫细胞化学检测,将培养的第3代细胞同Bio-oss胶原构建BMSCs/Bio-oss胶原复合物,构建小型猪下颌骨种植体周围骨缺损模型,将复合物植入骨缺损处,通过影像学、能谱分析种植体-新骨界面Ca2+含量及组织学检测初步评价其促成骨作用。结果Ⅰ型胶原、骨钙素免疫细胞化学染色呈阳性,Von Kossa染色可见钙结节形成;超微结构观察可见细胞生长附着于材料网孔内表面;8周时成骨组织学观察可见BMSCs/Bio-oss胶原复合物植入组成骨量大于Bio-oss胶原植入组,能谱分析显示,2组Ca2+含量有统计学差异。16周时,组织学观察可见2组成骨量已无明显差别,能谱分析显示,2组Ca2+含量无统计学差异。结论利用BMSCs/Bio-oss胶原复合物修复小型猪种植体周围骨缺损相对于植入Bio-oss胶原能够缩短种植体发生骨结合的时间。Objective To evaluate the effects of repairing bone defects around dental implants with tissue engineering bone estab lished with Biooss collagen scaffold. Methods Four minipigs were divided into 2 groups randomly. The bone marrow stroma cells （BMSCs） were separated with selfcontrol methods. They were cultured, proliferated and induced in vitro in osteogenic induction medi um. Collagen I and BGP were evaluated by immunoeytochemistry. The cultured passage 3 cells and Biooss collagen were mixed as BMSCs/Biooss collagen composite. Mandible periimplant bone defect model was established on minipigs. The composite was implan ted into bone defects. Contents of Ca2 ~ in the interface between implants and new bones were analyzed by imaging and energy disperse analysis. Make preliminary evaluation of its osteogenesis by histological examination. Results Staining of collagen I and BGP was positive by immunocytochemistry. Von Kossa staining proofed the formation of calcium nodules. Ultrastructure observation revealed the cells adhered to the inner meshed surfaces of the Biooss collagen. In the 8th week, new bone was more in BMSCs / Biooss collagen group than that in Biooss collagen group by histological observation. There was statistical difference between two groups in contents of Ca2＋ （P 〈0.05） according to energy disperse anaylsis. In the 16th week, there was no obvious difference in new bone quantities be tween two groups by histological observation, while there was no statistical difference in contents of Ca2＋ between two groups according to energy disperse anaylsis. Conclusions Using BMSCs/Biooss collagen composite to repair periimplant bone defects in minipigs can shorten the time for osseointegration of implants compared with using Biooss collagen alone.

关 键 词：骨缺损 种植体 Bio-oss胶原 骨组织工程 

分 类 号：R783.4[医药卫生—口腔医学]