人牙周膜细胞骨向分化中靶向调节PLAP-1基因的微小RNA的筛选与鉴定  

Screening and identification of miRNA regulating gene expression of PLAP-1 in osteoblastic differentiation of human peridontal ligament

作　　者：李长霞[1,2,3] 李春雷[4] 岳静[2,3] 黄欣[2,3] 陈美美[2,3] 高杰[2,3] 吴补领[2,3] 

机构地区：[1]广州军区联勤部门诊部口腔科,广东广州510515 [2]南方医科大学南方医院 [3]南方医科大学口腔医学院 [4]广东省人民医院口腔科

出　　处：《广东牙病防治》2013年第2期67-71,共5页Journal of Dental Prevention and Treatment

基　　金：广东省科技发展项目(2008B011300018-8);中国教育部研究生奖(20094433120004);广东省自然科学基金(8451051501000371)

摘　　要：目的筛选和鉴定靶向调节牙周膜相关蛋白1(peridontal ligament associated protein-1,PLAP-1)基因的微小RNA(microRNA,miRNA),探讨其在人牙周膜细胞骨向分化中的表达差异。方法用生物信息学方法筛选靶向调节PLAP-1基因的miRNA,用双荧光素酶法分析靶基因结合位点,定量PCR验证靶向调节PLAP-1基因的miRNA过表达及其在人牙周膜细胞骨向分化中的表达变化。结果 miR-21(miRNA-21)靶向调节PLAP-1,基因表达量在0 d时PLAP-1为1.001±0.053,miR-21为2.540±0.131;在28 d时PLAP-1为14.681±1.066,miR-21为1.000±0.016。过表达miR-21可抑制性调节靶基因PLAP-1表达,人牙周膜细胞骨向分化中PLAP-1的表达水平与miR-21的表达水平呈负相关。结论 miR-21靶向调节PLAP-1基因并参与人牙周细胞骨向分化中PLAP-1基因的表达调控。Objective To screen and identify miRNA which regulate gene expression of PLAP-1 and to analyze miR-NA differential expression patterns of PDLCs at various osteoblastic differentiation stages. Methods Bioinful＇nlatie analy-sis was performed to predict miRNA that potentially regulate gene expression of PLAP-1. Dual luciferase reporter assay and quantitative real time PCR were performed to confirm the effects of these miRNA on PLAP-1 gene expression. Re-suits miR-21 was targeted to regulate the expression of PI,AP-I. Overexpression of miR-21 reduced the expression level of PLAP-I. The expression level of PLAP-1 was inversely correlated with that of miR-21 during osteogenic differentiationof PDLCs. Conclusion Targeted regulation of miR-21 affects the expression of PLAP-1 during the osteogenic differentiation of PDLCs.

关 键 词：牙周膜 细胞分化 RNA 转录因子 基因表达 

分 类 号：R781.4[医药卫生—口腔医学]