MiR-26a与Smad1在垂体腺瘤中的作用机制体外研究  

Mechanism of MiR-26a and Smad1 in ACTH pituitary adenomas:in vitro study

作　　者：赵鑫[1] 王雪峰[1] 刘艳武[1] 薛丽[1] 徐延斌[1] 王智[1] 

机构地区：[1]哈尔滨医科大学附属第四医院微创神经外科,黑龙江哈尔滨150001

出　　处：《中华神经外科疾病研究杂志》2013年第1期41-44,共4页Chinese Journal of Neurosurgical Disease Research

基　　金：黑龙江省科技厅科技攻关项目资助项目(GC12C304-2);哈尔滨市科技局科技创新人才研究基金资助项目(2012RFXXS069);黑龙江省自然科学基金面上项目资助项目(D201257)

摘　　要：目的探讨垂体腺瘤中miR-26a与Smad1的关系以及对垂体腺瘤细胞生长以及激素分泌的影响。方法体外培养小鼠垂体腺瘤细胞(AtT-20),转染miR-26a抑制剂(miR-26a inhibitor),实时荧光定量多聚酶链反应检测Smad1 mRNA表达情况,Western blot检测Smad1蛋白的表达,双层软琼脂克隆形成实验分析细胞增殖情况,流式细胞技术检测细胞周期,电化学发光法检测培养基中促肾上腺皮质激素(ACTH)的浓度。结果抑制miR-26a后在垂体腺瘤中Smad1基因转录的mRNA没有明显变化,Smad1翻译的蛋白量增多,细胞增殖能力降低,停留在S期的细胞比例增多,ACTH分泌量下降。结论 Smad1是miR-26a的靶基因,miR-26a通过干扰Smad1 mRNA的翻译调控Smad1。miR-26a在ACTH型垂体腺瘤的生长以及激素分泌过程中起到重要作用。Objective To investigate the relationship between MiR-26a and Smadl in pituitary adenomas, and its effect on cell growth and hormone secretion of adrenecorticotropic hormone （ACTH）-secreting pituitary adenoma. Methods AtT-20 were in vitro cultured, and transfected with miR-26a inhibitor. Smadl mRNA expression in AtT-20 were detected by real time-polymerase chain reaction （PCR）. The expression of Smadl proteins was detected by Western blot. The cellular proliferation capacity was detected by the colony formation of the cells in soft agar medium. Mitotic cycle was detected by flow cytometry. The concentration of ACTH in medium was detected by method. Results There was no obvious change of Smadl mRNA after the inhibition of miR-26a. But protein translation of Smadl was increased, cell proliferation was decreased, cells in S phase cells were increased, and the secretion of ACIH was decreased. Conclusion Smadl is the target gene of miR-26a, miR-26a regulates the Srmadl mRNA translation to compass its ends. miR-26a plays an important role in the progression of cell growth and hormone secretion in ACid-secreting pituitary adenoma.

关 键 词：垂体腺瘤 miR-26a SMAD1 骨形态发生蛋白 促肾上腺皮质激素 

分 类 号：R739.4[医药卫生—肿瘤]