Cav3．2通道对背根节慢性压迫痛大鼠脊髓CaMKⅡ表达的影响  

THE EFFECTS OF Cav3.2 ON THE CaMK II EXPRESSION IN THE SPINAL CORD OF THE RATS FOLLOWED CHRONIC DORSAL ROOT GANGLIA COMPRESSION

作　　者：文先杰[1,2] 刘洪珍[1] 梁桦[1] 徐世元[2] 张庆国[2] 赖小红[1] 王汉兵[1] 杨承祥[1] 

机构地区：[1]佛山市第一人民医院麻醉科,佛山528000 [2]南方医科大学珠江医院,广州510282

出　　处：《中国疼痛医学杂志》2013年第3期134-138,共5页Chinese Journal of Pain Medicine

基　　金：佛山市卫生局科研项目(2013031);佛山市科技局医学重点攻关项目(No.201208080);国家自然科学基金青年项目(No.81100831);广东省医学科研基金项目(No.B2011303)

摘　　要：目的:观察Cav3.2通道对背根节慢性压迫痛大鼠脊髓CaMKⅡ表达的影响,探讨Cav3.2-CaMKⅡ通路在神经病理性疼痛中的作用。方法:雄性SD大鼠60只,体重250±20 g,随机分为5组,每组12只(其中行为学实验8只,免疫印迹实验4只)。分别是正常对照组(C组);模型组(CCD组);生理盐水组(NS组);错义寡聚核苷酸组(MS-Cav3.2组);反义寡聚核苷酸组(AS-Cav3.2组)。分别于鞘内置管前(T1),鞘内置管后3 d(T2),鞘内给药后1 d(T3)、4 d(T4),CCD模型制备后5d(T5)、10 d(T6)、15 d(T7)检测大鼠机械缩腿阈值(mechanical withdrawal threshold,MWT)和热缩足潜伏期(thermal withdrawal latency,TWL)。并于CCD模型制备后5 d,各组取4只大鼠处死,取脊髓腰膨大,采用免疫印迹法检测Cav3.2及CaMKⅡ的表达。结果:与C组比较,CCD组大鼠在模型制备后第5 d、10 d、15 d时MWT及TWL均显著降低。鞘内注射NS和Cav3.2错义寡聚核苷酸对CCD大鼠MWT及TWL无影响,鞘内注射Cav3.2反义寡聚核苷酸可增加CCD大鼠MWT和TWL,减轻大鼠机械痛敏和热痛敏。C组大鼠脊髓Cav3.2和CaMKⅡ蛋白均有表达。大鼠在制备CCD模型后5 d Cav3.2及CaMKⅡ蛋白表达均显著增加。鞘内注射NS及Cav3.2错义寡聚核苷酸对Cav3.2及CaMKⅡ蛋白表达无影响,鞘内注射Cav3.2反义寡聚核苷酸则可显著抑制Cav3.2及CaMKⅡ蛋白的表达。结论:抑制脊髓Cav3.2通道的表达可降低慢性背根节压迫痛大鼠脊髓CaMKⅡ的表达。Objective： To observe the effects of calcium chennel Cav3.2 on CaMK Ⅱ expression in the spinal cord of the rats following chronic dorsal root ganglia compression, we investigated the roles of Cav3.2-CaMK Ⅱ pathways in neuropathic pain. Methods： 60 male Sprague Dawley rats weighing 250 ＋ 20 g were randomly divided into 5 groups of 12 rats each, 8 rats for behavior experiment and 4 rats for western blot respectivly： Control group （group C）; Model group （CCD group）; Saline group （NS group）; Cav3.2 missense oligodeoxynucleotides group （MS-Cav3.2 group）; Cav3.2-antisense oligodeoxynucleotides group （AS-Cav3.2 group）. Mechanical withdrawal threshold （MWT） and thermal withdrawal latency （TWL） of the rats were measured before （T1） and 3 days after （T2） implantation of intrathecal catheters, 1 day （T3）, 4 d （T4） after drug or NS intrathecal injection, 5 d （T5）, 10 d （T6）, 15 d （T7） after CCD model preparation. Four rats in every group were executed and spinal cord were isolated to detect Cav3.2 and CaMK 1[ expression with western blot after 5 days of CCD model preparation. Results： Compared with C group, MWT and TWL of the rats in CCD group statistically decreased at T5, T6 and T7. There were no significant effects on the MWT or TWL after intrathecal injection of Saline or Cav3.2 miss-sense oligodeoxynucleotides. However, MWT and TWL of the rats significantly increased after anti-sense oligodeoxynucleotides injection. Cav3.2 and CaMKⅡ proteins were detected in the spinal cord of the rats in C group. Moreover, Cav3.2 and CaMKⅡ proteins were upregulated 5 days after CCD model preparation. There were no significant effects on the Cav3.2 and CaMK Ⅱproteins expression after saline and Cav3.2 miss-sense oligodeoxynucleotides intrathecal injection. However, Cav3.2 and CaMKⅡ proteins expression were significantly decreased after Cav3.2 anti-sense oligodeoxynucleotides injection. Conclusion： Inhibition of Cav3.2 expression can reduce CaMKⅡ expression

关 键 词：Cav3-2 CaMKⅡ 脊髓 背根节 神经病理性疼痛 

分 类 号：R741[医药卫生—神经病学与精神病学]