脊髓胶质细胞在rhEPO预防神经病理性疼痛中的作用机制的研究  

THE MECHANICAL STUDY OF RECOMBINANT HUMAN ERYTHROPOIETIN ON A RAT MODEL OF NEUROPATHIC PAIN

作　　者：贾宏彬[1] 刘健[1] 李伟彦[1] 徐建国[1] 金毅[1] 

机构地区：[1]南京军区南京总医院麻醉科疼痛医学中心,南京210002

出　　处：《中国疼痛医学杂志》2013年第3期164-167,共4页Chinese Journal of Pain Medicine

基　　金：南京军区医学科学技术研究"十一五"计划重点课题;课题号(06Z39)

摘　　要：目的:探讨预防性给予重组人红细胞生成素对雄性大鼠L5脊神经切断大鼠机械、热痛觉过敏的影响及可能的机制。方法:选用Sprague-Dawley(SD)雄性大鼠30只,选用L5脊神经切断神经病理性模型;随机分成3组(n=10):假手术+生理盐水组、损伤+生理盐水组、损伤+重组人促红细胞生成素(recombinant human erythropoietin,rhEPO)(5000 U/kg)组;术前1天给药,连续给药7天。采用von Frey纤维和Hargreaves法测定各组机械缩足反射阈值(mechanicalwithdrawal threshold,MWT)和热缩足潜伏期(thermal withdrawal latency,TWL);采用实时定量多聚酶链反应(real-time polymerase chain reaction,RT-PCR)的方法检测脊髓胶质细胞标志物白细胞分化抗原11b(cluster of differentiation antigen 11b,CD11b)和胶质细胞酸性蛋白(glial fibrillaryacidic protein,GFAP)的mRNA表达水平。结果:与生理盐水预给药手术组相比,预防性腹腔注射rhEPO 5000 U/kg,能显著缓解大鼠的机械、热痛高敏行为(P<0.05,P<0.01),降低脊髓胶质细胞白细胞分化抗原和胶质细胞酸性蛋白的mRNA表达水平(P<0.05)。结论:发现预防性给予rhEPO能预防神经病理性疼痛的发生。其效应与其抑制胶质细胞活性相关。Objective： To investigate the effect of rhEPO administrated intraperitoneally before or after nerve injury on the behavioral manifestation and the activation of glial cell in spinal cord using a rat model of neuropathic pain induced by L5 nerve transection. Methods： The thirty male Sprague-Dawley （SD） rats were randomly assigned into three groups： sham-operation with saline, L5 spinal nerve transection with saline and L5 spinal nerve transection with rhEPO （5000 U/kg ）. rhEPO or saline was given intraperitoneally 1 day before surgery and continued daily to day 7 post-transection. The hind paw mechanical withdrawal threshold （MWT） and thermal withdrawal latency （TWL） of all animals were measured on day 7 after operation with von Frey hairs and Hargreaves tests, respectively. The transcription of mRNA of glial cell surface markers such as CDllb and GFAP was semi-quantitatively evaluated using real-time PCR assay. Results： Administration of rhEPO resulted in attenuation in mechanical and thermal hyperalgesia. Furthermore, rhEPO could markedly inhibit the level of mRNA expression of CDllb and GFAP in the spine cord （P 〈 0.05）. Conclusion： We found the potential preventive value of rhEPO in the treatment of neuropathic pain, which correlated with the decreased spinal cord mRNA expression of CD1 lb and GFAP.

关 键 词：神经病理性疼痛 人红细胞生成素 痛觉高敏 白细胞分化抗原11b 胶质细胞酸性蛋白 

分 类 号：R741[医药卫生—神经病学与精神病学]