胶体金对MC3T3-E1增殖、分化及RUNX2基因表达的影响  

Effect of gold colloidals on proliferation,differentiation and expression of RUNXl in MC3T3-E1 cells

作　　者：姚元元[1] 陈凤山[1] 陈玉成[1] 

机构地区：[1]同济大学附属口腔医院正畸科,上海200072

出　　处：《同济大学学报（医学版）》2013年第1期35-39,共5页Journal of Tongji University(Medical Science)

摘　　要：目的研究30 nm胶体金对小鼠成骨细胞系MC3T3-E1增殖、分化及成骨相关基因RUNX2表达的影响。方法体外培养小鼠成骨细胞MC3T3-E1,在α-MEM培养液中加入不同体积的胶体金处理细胞,利用MTT法检测胶体金对成骨细胞增殖活性的影响,碱性磷酸酶活性测定及RT-PCR法分别检测其对成骨细胞分化及成骨相关基因RUNX2表达的影响。结果浓度为10^(-10)、10^(-11)、10^(-12) mg/L的胶体金均促进成骨细胞的增殖,且ALP相对活性及RUNX2基因表达也均高于阳性对照组。结论 30 nm胶体金能促进小鼠前成骨细胞系MC3T3-E1增殖、分化及成骨相关基因RUNX2的表达,进而促进成骨。Objective To investigate the effect of gold colloidals （Au NPs） on proliferation, differentiation and expression of runt-related transcription factor 2 （Runx2） in MC3T3-E1 cells. Methods Mouse calvarial preosteoblasts （MC3T3-E1） were cultured on alpha minimum essential medium （a-MEM） and treated with different concentrations of gold colloidals. The cell proliferation was evaluated by MTT method, cell differentiation was assessed by measuring alkaline phosphatase （ALP） activity and the expression of RUNX2 mRNA was detected by reverse transcriptase polymerase chain reaction （RT-PCR）. Results Gold colloidals at concentrations of 10^（-10）、10^（-11）、10^（-12） mg/L promoted cell proliferation and differentiation and enhanced the expression of RUNX2 mRNA in MC3T3-E1 cells. Conclusion Results indicate that gold colloidals can enhance cell proliferation and osteogenic differentiation and Runx2 expression in MC3T3-E1, indicating that it may have potential to promote bone regeneration.

关 键 词：胶体金 MC3T3-E1 增殖 分化 RUNX2基因 小鼠 

分 类 号：R781.4[医药卫生—口腔医学]