乳腺癌中法尼酯X受体的表达及意义  

Expression of FXR mRNA in breast cancer and its significance

作　　者：慈思慧[1] 张瑰红[1] 曹渊[1] 吴正升[1] 杨枫[1] 徐晓春 

机构地区：[1]安徽医科大学病理学教研室,合肥230032 [2]M.D.Anderson Cancer Center,University of Texas,USA

出　　处：《临床与实验病理学杂志》2013年第3期253-256,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：安徽省高校省级自然科学研究重点项目(KJ2011A168)

摘　　要：目的观察法尼酯X受体(farnesoid X receptor,FXR)在人乳腺癌组织中的表达,并分析其与患者临床病理参数以及ERα、ERβ之间的关系。方法采用RQ RT-PCR技术检测25例新鲜乳腺良性增生组织和60例乳腺浸润性导管癌组织中FXR mRNA的表达水平,并分析其与患者临床病理参数以及ERα、ERβmRNA表达的关系。结果乳腺浸润性导管癌组织中FXR mRNA相对表达水平(9.7±2.7)明显低于良性增生组织(40.8±7.9)(P=0.007);淋巴结转移组FXR mRNA相对表达水平(17.1±9.0)明显高于无淋巴结转移组(4.3±2.9)(P=0.041);乳腺浸润性导管癌组织中FXR mRNA的相对表达水平与ERβ呈正相关(P=0.000,r=0.771),与ERα无相关性(P=0.051)。结论 FXR可能参与乳腺癌的发病,其高水平表达时可能促进乳腺癌的侵袭转移;FXR与ERβ两者表达呈正相关。Purpose To investigate the expression of FXR mRNA and their relationships with ERa, ERl3and clinicopathologic charac- ters in breast carcinoma. Methods Real time quantitative transcription polymerase chain reaction （ RQ RT-PCR） technique was used to detect FXR mRNA expression in 60 cases of invasive breast carcinoma and 25 cases of breast benign lesions. Result The RQ RT- PCR data showed that FXR expression was reduced in invasive breast carcinoma tissues （9. 7 ± 2. 7 ） compared with breast benign le- sions tissues （40. 8 ±7.9 ） （P = 0. 007, respectively）. Furthermore, FXR mRNA expression was significantly higher in breast carcino- mas with lymph node positive （ 17. 1± 9. 0） than thoses with lymph node negative cases （4. 3 ± 2. 9 ） （P = 0. 041 ）. The expression of FXR mRNA was positively correlated with the expression of ERβ （P = 0. 000, r = 0. 771 ） in invasive breast carcinoma tissues but was not associated with the expression of ERa （ P = 0. 051 ）. Conclusion The data from the current study show that FXR may play various roles in the carcinogenesis of breast cancer. FXR mRNA express at high levels may contribute to lymph node metastasis in breast canc- er. The results of this study also indicate that the expression of FXR is positively correlated with the expression of ERβin invasive breast carcinoma.

关 键 词：乳腺肿瘤 法尼酯X受体 转移 逆转录聚合酶链反应法 

分 类 号：R737.9[医药卫生—肿瘤] R394[医药卫生—临床医学]