乌骨藤制剂诱导人肝癌细胞Bel-7402凋亡的实验研究  

Experimental study on effect of Glaucescent Fissistigma Root preparation on Bel-7402 cells apoptosis

作　　者：张锐[1] 刘静 刘阳晨 周绍兵 叶宏勋 崔永安[2] 

机构地区：[1]扬州大学附属泰兴人民医院肿瘤放疗科,江苏泰兴225400 [2]泰州市人民医院肿瘤科,江苏泰州225300

出　　处：《现代肿瘤医学》2013年第3期488-491,共4页Journal of Modern Oncology

摘　　要：目的:探讨乌骨藤制剂对人肝癌细胞Bel-7402株的影响并初步探讨其机制。方法:分别用不同浓度的乌骨藤制剂处理人肝癌细胞Bel-7402,未经药物处理的细胞为阴性对照组,采用MTT法检测肝癌Bel-7402细胞增殖、流式细胞术检测人肝癌细胞Bel-7402的凋亡情况以及p53蛋白的表达。结果:不同浓度的乌骨藤制剂(10mg/ml,20mg/ml,40mg/ml)处理肝癌Bel-7402细胞后,其增殖受到抑制,作用24h的抑制率分别为11.57%、21.09%、31.27%,48h的抑制率为27.55%、35.95%、45.13%,随着药物浓度的增加(20mg/ml,40mg/ml,80mg/ml)细胞凋亡显著增加,凋亡率分别为(12.2±0.67)%、(17.4±0.69)%、(22.9±1.38)%,而p53蛋白的表达也随着药物浓度的增加而增加。结论:乌骨藤制剂能够抑制人肝癌细胞的增殖,诱导其凋亡,作用机制可能与激活p53基因有关。Objective：To study the effect of Glaucescent Fissistigma Root preparation on Be1-7402 cells and its mechanisms. Methods：After being treated by Glaucescent Fissistigma Root injection of different concentrations, untre- aed cells as control, viability of Bel - 7402 cells was determined by MTI method, apoptosis and p53 protein assessed by FACS. Results：The inhibition（ 11.57% ,21.09% and 31.27% in 24h,27.55% ,35.95% and 45.13% in 48h） was observed while the cells were treated by different concentrations of Glaucescent Fissistigma Root injection. Also, the apoptosis [ （ 12.2 ±0.67） %, （ 17.4 ＋0.69） % （22.9 ±1.38） % ] and the increasing of p53 protein was detec- ted ＇after treated by the injection（20mg/m1,40mg/ml,80mg/ml）. Conclusion：Glaucescent Fissistigma Root injection could inhibit the proliferation of Bel -7402 cells and induce apoptosis,the mechanisms may be related with p53 gene being activated.

关 键 词：乌骨藤 肝癌细胞 凋亡 P53 

分 类 号：R73-34[医药卫生—肿瘤] R735.7[医药卫生—临床医学]