大鼠颞叶癫痫点燃后海马zif268mRNA及其蛋白的时空表达变化  

Temporospatial expression of zif268mRNA and Zif268 protein in hippocampus in rats after kindling of temporal lobe epilepsy

作　　者：李春女[1] 李淞[2] 李秀杰[1] 张淑琴[3] 梁建民[1] 

机构地区：[1]吉林大学白求恩第一医院儿科,吉林长春130021 [2]吉林省人民医院神经内科,吉林长春130021 [3]吉林大学白求恩第一医院神经内科,吉林长春130021

出　　处：《中风与神经疾病杂志》2013年第2期141-143,共3页Journal of Apoplexy and Nervous Diseases

基　　金：中国博士后基金(2005037033);吉林省自然科学基金(201015147);长春市科技局儿科诊疗中心重点专科建设项目(2010120)

摘　　要：目的探讨海马zif268mRNA及其蛋白的时空表达变化与颞叶癫痫脑损伤的关系。方法将雄性Wistar大鼠随机分为3组:其中正常组6只,假手术对照组(Sham组)和海人酸(KA)颞叶癫痫点燃组(TLE组)各36只,后两组按点燃后6h、24h、3d、7d、14d、21d时间点各分为6小组,每小组6只。采用KA杏仁核点燃建立经典颞叶癫痫模型,应用原位杂交和免疫组织化学方法分别检测海马神经元zif268mRNA及其蛋白的表达。结果TLE组zif268mRNA表达在总体上和点燃后远期21d海马CA1、CA3区和齿状回(DG)均高于Sham组(P<0.05)。TLE组Zif268蛋白表达在总体上和远期21d海马DG表达低于Sham组(P<0.05)。回归分析提示颞叶癫痫与海马zif268mRNA表达呈正相关(β=0.286,P<0.001),与Zif268蛋白表达呈负相关(β=-0.153,P<0.001)。结论颞叶癫痫大鼠海马zif268mRNA及其蛋白的时空表达变化可能参与颞叶癫痫发病及其脑损伤过程。Objective To explore the relationship of the temporospatial expression of zif268mRNA and its protein in hippocampus with the epileptogenous brain injury in temporal lobe epilepsy （TLE）. Methods Male wistar rats were ran- domly divided into three groups：six were in normal group, 36 rats were in each of the other two groups, sham group and kainic acid （KA） kindled TLE group （TLE group） , and were subdivided into 6 subgroups according to the time points of 6h ,24h,3d,Td, 14d,21d after KA kindling, respectively. Each subgroup had 6 rats. Typical temporal lobe epileptic model was established by microinjeeted KA into right nucleus amygdalae in rats. In situ hybridization and immunohistochemistry method were used to spatio-temporally observe the expression of zif268mRNA and their protein in hippoeampus in rats after kindling or sham operating, respectively. Results In general or 21d after KA kindling, zif268mRNA were highly expressed in CA1 , CA3 and DG regions in hippocampus in TLE group than in that of sham group（P 〈 0.05 ）. On the whole,the expres- sion of Zif268 protein was significantly decreased than that of sham group （P 〈 0.05 ） , and it was same on 21d after KA kindling （ P 〈 0.05 ）. Regression analysis showed that the expression of zif268mRNA in hippoeampus was positively, correla- ted with TLE epileptogenesis （ β= 0.286 ,P 〈0. 001 ） ,while the Zif268 protein was negatively correlated （ β= -0. 153 ,P 〈 0. 001 ）. Conclusion The spatio-temporal changes of the expression of zif268mRNA and Zif268 protein may be involved in the mechanisms underlying TLE and subsequent brain injury.

关 键 词：颞叶癫痫 海人酸 点燃 zif268 

分 类 号：R742.1[医药卫生—神经病学与精神病学]