立枯丝核菌遗传多样性的研究方法  

Methodology in Researching of Genetic Variance of Rhizoctonia solani

作　　者：王利红[1,2] 姜华[2] 王艳丽[2] 孙国昌[2] 

机构地区：[1]杭州师范大学生命与环境科学学院,杭州310036 [2]浙江省农业科学院植物保护与微生物研究所,杭州310021

出　　处：《中国生物化学与分子生物学报》2013年第3期226-233,共8页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金(No.31071644);转基因生物新品种培育重大专项(No.2011ZX08009-003-001)资助~~

摘　　要：立枯丝核菌(Rhizoctonia solani Kühn)是一个集合种,遗传多样性丰富.关于遗传多样性的研究一直是R.solani研究的热点.本文就用于R.solani遗传多样性研究的方法进行了综述.分别解释并阐述了各种方法的优缺点,其中菌丝融合法是研究R.solani遗传多样性的传统方法,该法需借助显微镜且耗时耗力;同工酶法简便、高效、低廉,但常需要与其它方法联用;脂肪酸法操作难度小,价格相对较低,但该法受菌株生长状况和脂肪酸脂化方法的限制;分子标记法方法众多,各有利弊,通过比较发现rDNA-ITS是研究R.solani遗传多样性比较合适的方法.本文还介绍了不同方法在R.solani遗传多样性研究中的具体应用.Rhizoctonia solani Kuhn is a plant pathogenic fungus with a wide host range and abundant genetic diversity. The study on the genetic diversity of R. solani is a kind of research hotspot. In this paper, we reviewed several techniques widely used in the studies on the genetic diversity of R. solani. We interpreted and described the advantages and disadvantages of each method. Hyphal fusion is a traditional method, which requires the use of a microscope and is time-consuming and labor-intensive. The method for identifying the pattern of isoenzyme is simple, efficient and inexpensive, and has to be associated with other techniques. The fatty acids analysis is easy to be manipulated, and the cost is relatively low, but the method is limited by the strain growth conditions and the way of fatty acid esterified. Many molecular marker methods were used in the research on the genetic diversity of R. solani, each with the pros and cons, by comparison we suggest that the rDNA-ITS is the more appropriate method. The applications of these methods were also discussed in the paper.

关 键 词：立枯丝核菌 菌丝融合 同工酶分析 脂肪酸分析 分子标记法 遗传多样性 

分 类 号：Q939.5[生物学—微生物学]