海洋曲霉属真菌菌株F5抑菌活性代谢产物的分离与鉴定  

Isolation and structural elucidation of the antibacterial metabolites produced by marine-derived Aspergillus strain F5

作　　者：李莹[1] 李进军[2] 王辂[2] 叶波平[1] 

机构地区：[1]中国药科大学生命科学与技术学院,南京210009 [2]抗生素研究与再评价四川省重点实验室,中国医药集团总公司四川抗菌素工业研究所,成都610052

出　　处：《中国抗生素杂志》2013年第3期191-194,199,共5页Chinese Journal of Antibiotics

基　　金：国家自然科学基金资助项目(NO 81072571)

摘　　要：目的从一株分离自广东湛江特呈岛木榄根际土壤的曲霉属真菌F5的发酵液中分离抑菌活性次级代谢产物。方法发酵液经乙酸乙酯萃取、正相硅胶柱层析、分子筛层析、C18柱制备色谱分离获得单体,并对化合物进行结构鉴定及活性测试。结果从菌株F5的乙酸乙酯提取物中分离得到2个内酯、2个异香豆素类化合物和1个不饱和酸,根据化合物的谱学特征(ESI-MS、1H-NMR和13C-NMR)和理化性质分别鉴定为(1)asperlactone、(2)aspyrone、(3)penicillic acid、(4)cis-4-hydroxymellein和(5)trans-4-hydroxymellein。其中化合物4在终浓度50μg/mL时,对Candida albicans有抑制作用。结论菌株F5分泌的代谢产物cis-4-hydroxymellein(4)对Candida albicans有明显抑制作用。Objective To identify and purify the antibacterial metabolites from the fermentation broth of Aspergillus sp. F5, isolated from the rhizospheric soil of Bruguiera gymnorrhiza, Guangdong. Methods Fermentation broth was extracted with acetic ether, then the extraction was separated and purified by silica gel, Sephadex LH20 and RP-18 colunm chromatography. Their structures were elucidated by means of spectral analysis including ESI-MS, 1H-NMR and 13C-NMR. Their antibacterial activity were detected with Alamar-Blue method. Results Five compounds were separated and purified from the acetic ether extraction of this Aspergillus strain, two lactones, two isocoumarin compounds and a unsaturated acid. Their structures were elucidated as （1） Asperlactone, （2） Aspyrone, （3） Penicillic acid, （4） cis-4-hydroxymellein and （5） trans-4-hydroxymellein by spectroscopic analysis（ESI- MS, 1H-NMR and ^13C-NMR）, among which compound 4 showed apparently antifungal activity against the growth of Candida albicans in the final concentration of 50μg/mL. Conclusion Strain F5 secretes cis-4-hydroxymellein（4） which effectively inhibits the growth of Candida albicans.

关 键 词：曲霉属 次级代谢产物 分离纯化 结构鉴定 抑菌活性 

分 类 号：Q949.327.1[生物学—植物学]