泛耐药鲍曼不动杆菌基于管家基因和水平转移基因的菌株亲缘性分析  

Phylogenetic analysis based housekeeping genes and horizontal transfer resistance genes in pandrug-resistant Acinetobacter baumannii

作　　者：耿先龙[1] 王春新[1] 许亚丰[1] 陈国千[1] 赵琪[1] 周丽珍[1] 糜祖煌[2] 金辉[3] 

机构地区：[1]南京医科大学附属无锡人民医院医学检验科,无锡214023 [2]无锡市克隆遗传技术研究所,无锡214026 [3]东南大学公共卫生学院流行病与卫生统计教研室,南京210096

出　　处：《中国抗生素杂志》2013年第3期I0005-I0008,共4页Chinese Journal of Antibiotics

基　　金：无锡市医院管理中心科技发展基金项目(YGM1001)

摘　　要：目的调查20株泛耐药鲍曼不动杆菌(pandrug-resistant Acinetobacter baumannii,PDR-ABA)的菌株亲缘性。方法收集2010年3月到2010年5月临床标本中分离的PDR-ABA菌20株,采用聚合酶链反应(PCR)的方法检测3种管家基因和37种β-内酰胺类获得性耐药基因、15种氨基糖苷类获得性耐药基因、5种喹诺酮类获得性耐药基因和8种可移动遗传元件等水平转移基因检测,并对检测结果作了样本聚类分析。结果本组PDR-ABA菌carO、gyrA和parC3种管家基因均存在突变,且突变形式一致;blaTEM、blaADC-like、blaOXA-23、aac(6')-Ⅰb、ant(2")-Ⅰ、ant(3")-Ⅰ、aphA1、adeB、tnpU、ISCR1、IS26和ISaba1基因全部阳性,blaCARB-2、blaDHA-1和IS903基因阳性率为5%。样本聚类分析显示本组PDR-ABA菌为克隆传播。结论本组PDR-ABA菌为医院内克隆传播,应加强控制。Objective To investigate the phylogeny of 20 strains of pandrug-resistant Acinetobacter baumannii （PDR-ABA）. Methods From March 2010 to May 2010, 20 strains of PDR-ABA were collected from clinical specimen. Then, 3 kinds of housekeeping genes, 57 kinds of acquired resistance-related genes to beta-lactams, aminoglycosides, quinolones and 8 kinds of genetic markers of mobile genetic element were analyzed by PCR and the phylogenetic analysis was performed. Results All 20 strains of PDR-ABA were existed mutations for 3 kinds of housekeeping genes and positive for blarEM, b[anoc.like, blaoxn.23, aac（6＇）- I b, ant（2 ＂）- I, ant（3 ＂）- I, aphA1, adeB, tnpU, ISCR1, IS26, 1Sabal, and the positive rate of genes of blaCARB-2, blaDKA.1 and IS903 were 5.0%. Sample cluster analysis showed that this group of PDR-ABA were clonal spread. Conclusions This group of PDR-ABA were clonal spread and the outbreak should be controlled.

关 键 词：鲍曼不动杆菌 亲缘性分析 泛耐药 

分 类 号：R378.1[医药卫生—病原生物学]