牛蒡子苷元对肿瘤血管生成抑制作用的观察  

Inhibition effect of arctigenin on the angiogenesis of hepatocarcinoma

作　　者：郑国灿[1] 王兵[1] 黄聪华[1] 李国明[1] 李叔强[1] 袁家天[1] 

机构地区：[1]东南大学医学院附属中大医院普外科,江苏南京210009

出　　处：《中华肿瘤防治杂志》2013年第3期177-180,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：东南大学国家自然基金预研基金(XJ0650261)

摘　　要：目的:观察牛蒡子苷元(ARG)对裸鼠体内、体外肿瘤血管生成的影响,并探讨其作用机制。方法:利用人肝癌SMMC-7721细胞进行体内、外实验。体外实验分别采用RT-PCR、细胞免疫组化检测ARG作用前后SMMC-7721细胞中血管内皮生长因子(VEGF)基因及蛋白的表达;体内实验建立裸鼠SMMC-7721细胞肺转移瘤模型,成瘤后ARG干预,第30天取出肿瘤组织,免疫组化检测裸鼠瘤体CD34标记的血管内皮细胞,计算微血管密度(MVD)。结果:VEGF mRNA表达量经ARG及5-FU作用后,阴性对照组、3个药物组及阳性对照组依次为1.01±0.03、0.92±0.02、0.81±0.15、0.72±0.03和0.82±0.26;实验组VEGF蛋白表达率为44.16%,明显低于对照组的82.13%。ARG作用后VEGF mRNA及蛋白表达水平下降,P<0.05。裸鼠SMMC-7721细胞肺转移瘤的MVD减少,空白对照组、药物组和5-FU组裸鼠MVD值分别为39.43±3.31、19.29±2.06和21.57±2.82,P<0.01。结论:ARG通过下调裸鼠VEGF mRNA及蛋白的表达,抑制裸鼠SMMC-7721细胞肺转移瘤的血管生成。OBJECTIVE： To explore the effect of Arctigenin（ARG） on the angiogenesis of hepatocarcinoma in nude mice in vivo and vitro and its significance. METHODS： Expression levels of VEGF mRNA and protein in SMMC-7721 cell were determined by reverse transcription polymerase-chain-reaction （RT-PCR） and respectively in vivo. The hepatocarci- noma cells were inoculated into subcutaneous of the nude mice. The tumors were taken out on the 30th day after the thera- py of Arctigenin. The difference at the level of protein of gene of VEGF was observed by immunohistochemistry between the experimental group and control group, the SMMC-7721 cells metastatic tumor＇s MVD in vivo groups were calculated. RESULTS： The expression of VEGF mRNA in the experimental group was weaker than that in control group（P〈0.05）, and the expression of VEGF mRNA of the different groups were 1.01±0.03,0. 92±0.02,0. 81±0.15,0. 72±0.03 and 0. 82±0. 26. The luminance ratio of VEGF gene in the experimental group was lower than that in the control group （44.16% vs 82.13%）. MVD in the experimental group （19. 29±2.06） was lower than that in the blank control group （39. 43±3.31） and 5-FU group （21. 57±2.82 ,P〈0. 01）. CONCLUSION： Arctigenin can inhibit the expression of VEGF gene and protein in hepatocarcinoma cell and the angiogenesis of hepatocarcinoma in nude mice.

关 键 词：肝肿瘤 牛蒡子苷元 SMMC-7721细胞 新生血管化 病理性 血管内皮生长因子 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤]