胃癌组织Flt-1和PDGFR及bFGFR表达与MVD相关性研究  

Expressions of Flt-1,PDGFR,bFGFR and MVD in gastric tissues and their relationship

作　　者：张君红[1] 林瑶光[1] 

机构地区：[1]广西医科大学第一附属医院老年消化内科,广西南宁530021

出　　处：《中华肿瘤防治杂志》2013年第4期269-272,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：广西科技厅科研基金(0342010-4)

摘　　要：目的:探讨血管内皮生长因子受体-1(Flt-1)、血小板衍生生长因子受体(PDGFR)、碱性成纤维生长因子受体(bFGFR)及微血管密度(MVD)的表达及相关性研究。方法:分别选择71例手术切除的胃癌及39例正常胃组织石蜡标本为观察组及对照组,应用免疫组织化学方法检测组织中Flt-1、PDGFR和bFGFR的蛋白表达及MVD。结果:Flt-1、PDGFR和bFGFR在胃癌组织中的阳性率分别为53.5%、49.3%和50.7%,高于对照组(12.8%、10.3%、7.7%),χ2值分别为17.514、16.765和20.350,P值均为0.000。且上述受体的表达与胃癌的TNM分期、浸润及淋巴结、远处转移密切相关,P<0.01或P<0.05。胃癌组MVD(45.2±15.2)高于对照组(28.6±4.2),t=5.083,P=0.001。MVD与肿瘤的TNM分期、浸润深度、淋巴结和远处转移密切相关,P<0.01。3种受体在胃癌中的表达存在正相关,P<0.01。3种受体在胃癌组织中表达均阳性伴有淋巴结转移的发生率为91.7%,高于三者表达均阴性者(68.2%),χ2=4.023,P=0.045;向远处转移的发生率分别为58.3%和18.2%,χ2=7.769,P=0.005。三者表达均阳性和均阴性的胃癌组织中MVD计数分别为60.5±18.0和40.4±5.1,两组比较差异有统计学意义,t=21.38,P=0.001。结论:3种受体在胃癌组织中表达增高,且与MVD有相关性,它们在胃癌的发生、发展、浸润及转移中可能存在相互诱导及协同作用。OBJECTIVE： To investigate the relationship of vascular endothelial growth factor receptor（Fit-I） ,plate- let-derived growth factor receptor（PDGFR）, basic fibroblast growth factor receptor （bFGFR） and angiogenesis to the bio- logical behavior of gastric carcinoma（GC）. METHODS： Seventy-one cases of GC,and 39 cases of normal gastric tissues were examined by using immunohistochemistry for the expression of angiogenic factor receptor and microvessel density （MVD）. RETULTS.. The positive rates of Flt-1 ,PDGFR and bFGFR in GC tissues were 53.5% ,49.3% and 50.7% re- spectively, and were significantly higher than that in patients with normal tissues（12.8 %, 10.3 %, 7.7 %, X2 were 17. 514, 16. 765 and 20. 350,P= 0. 000）. The high positive expression of three receptors in GC group was significantly correlated with TNM stage,regional invasion,lymphatic and blood stream metastasis（P〈0.01 or P〈0.05）. But there were no cor- relation with the tumor size,histology type and differentiation degree. The MVD counts in GC and normal tissues were 45.2±15.2 and 28.6±4.2 respectively（t= 5. 083,P=0. 001） ,the high positive expression of MVD had correlation with TNM stage, regional invasion and lymphatic and blood stream metastasis in GC（P〈0.01）. There were significant corre- lation among the expression of Fit-1 ,PDGFR and bFGFR（P〈0.01）. The incidence rates of lymph mode metastasis and blood metastasis with all three angiogenic factor receptors positively expressed, or all three negatively expressed were 91.7% and 68. 2%,58. 3% and 18. 2%,respectively（X2 =7. 769,P=0.005）. The MVD counts in GC tissues were 60.5 ± 18.0,40.4 ±5. 1 （t = 21.38, P = 0.001 ）. CONCLUSIONS.. The expression of three angiogenesis factor receptors and MVD might be significantly correlation with growth,invasion and metastasis of GC. The three receptors together may play a coordinated role in metastasis and invasion.

关 键 词：胃肿瘤 血管生长因子受体 微血管密度 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]