人肺腺癌组织和细胞系TROP-2表达的初步研究  

Primary study of expression of TROP-2 in human pulmonary adenocarcinoma and cell lines

作　　者：顾宇平[1] 赵云根[2] 朱一蓓[3] 张光波[3] 黄建安[1] 

机构地区：[1]苏州大学附属第一医院呼吸科,江苏苏州215006 [2]常熟市第二人民医院呼吸科,江苏常熟215500 [3]苏州大学附属第一医院临床免疫实验室,江苏苏州215006

出　　处：《中华肿瘤防治杂志》2013年第5期342-346,共5页Chinese Journal of Cancer Prevention and Treatment

基　　金：常熟市科技计划社会发展项目(CS201219)

摘　　要：目的:检测人滋养层细胞表面抗原-2(TROP-2)在人肺腺癌组织和细胞系中的表达,探讨其与临床病理因素和预后的关系。方法:流式细胞术检测5株人肺腺癌细胞系、1株永生化正常人支气管上皮细胞系中TROP-2的表达,RT-PCR检测11例、免疫组化检测46例肺腺癌组织中TROP-2的表达,并结合临床病理因素及预后进行分析。结果:人肺腺癌细胞系中H1975、SPCA-1和PC-9有TROP-2表达,表达阳性率分别为(45.3±5.4)%、(91.4±4.6)%和(97.2±2.1)%,而在A549、H1299及正常人支气管上皮细胞系HBE中无表达。TROP-2 mRNA的表达阳性率为72.7%(8/11)。TROP-2蛋白在肺腺癌组织中的阳性率(43.5%,20/46)明显高于癌旁正常肺组织(0,0/11)和肺良性病变组织(9.5%,2/21),P=0.001。肺腺癌TROP-2的表达与肿瘤淋巴结转移(P=0.02)和TNM分期(P=0.02)相关,与患者术后生存期呈负相关趋势,但差异无统计学意义,P=0.068。结论:TROP-2在肺腺癌组织及肺腺癌细胞系中均有较高表达,可能在肺腺癌发生发展中发挥重要作用。OBJECTIVE.. To investigate the expression of TROP2 in human pulmonary adenocarcinoma （ADC） tis sues and cell lines. METHODS： TROP2 expression was detected by flow cytometry in five ADC cell lines and one immor talized human bronchial epithelial cell line HBE. TROP2 mRNA was examined in 11 patients with ADC and also analyzed immunohistochemically in 46 patients with ADC. Furthermore, the correlations between TROP2 and elinieopathologic fea tures and prognosis of ADC were analyzed. RESULTS： TROP2 was detected in H1975,SPCA1 and PC9,but not in A549,H1299 and HBE. The expression of TROP2 in H1975,SPCA1,PC9 respectively were （45.35：5.4）%, （91.4± 4.6） % and （97.24±2.1）%. TROP2 mRNA expression in ADC tissues detected by RTPCR was 72.7%（8/11）. TROP2 expression detected immunohistochemieally was significantly increased in ADC tissues （43. 5%, 20/46） than that in tumor adjacent normal tissues （0） and in benign lesions （9.5 %, 2/21,P= 0. 001）. The overexpression of TROP2 was correlated with lymph node metastasis （P=0.02） and TNM stage （P= 0.02）. TROP2 tended to be expressed in cases with an unfavorable outcome （P~ 0. 068）. CONCLUSION.. TROP2, which is highly expressed in pulmonary ADC tissues and cell =ines.may be involved in carcinogenesis.

关 键 词：肺腺癌 TROP-2 流式细胞术 RT-PCR 免疫组化 

分 类 号：R734.2[医药卫生—肿瘤]